当前位置: X-MOL 学术Ann. Appl. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Banana defence responses to Cosmopolites sordidus feeding and methyl jasmonate application
Annals of Applied Biology ( IF 2.6 ) Pub Date : 2020-08-26 , DOI: 10.1111/aab.12638
Elyeza Bakaze 1, 2 , Beloved Mensah Dzomeku 3 , Jens‐Norbert Wünsche 1
Affiliation  

Each year 25–75% of banana and plantain yields are lost because of rhizome damages caused by banana weevil (Cosmopolites sordidus) in growing regions of sub‐Saharan Africa. However, the specific plant defence response of the rhizome tissue in relation to the C. sordidus attack is unknown. Consequently, in this study, we evaluated whether plant defence substances in the rhizome are correlated with the degree of larval damage and whether applications of methyl jasmonate (MJ) elicit a greater induction of the plant defence potential against C. sordidus. Moreover, we attempted to reveal cellular modifications in response to the root feeding herbivore through histochemical staining. The banana cultivars “Km5” and “Mbwazirume” with tolerance and susceptibility to C. sordidus, respectively, were used in a pot experiment to evaluate percent rhizome damage, leaf chlorophyll content, total phenolic content (TPC), antioxidant capacity and cell morphology in response to C. sordidus attack and/or MJ applications compared to untreated control plants. We found that C. sordidus‐induced rhizome damage was 30% in the susceptible cultivar but less than 5% in the tolerant cultivar. The percent rhizome damage was not related to leaf chlorophyll content but showed a significant negative linear relationship to both TPC and antioxidant capacity. Larvae feeding induced a considerably greater increase of polyphenolic defence compounds in Km5 than in Mbwazirume; however, this response was opposite in the MJ treatment, suggesting that the phytohormone induced the susceptible plant to invest more into the synthesis of defence chemicals that in turn lead to reduced C. sordidus damage. Tissue staining demonstrated a greater deposition of lignin and suberin in C. sordidus challenged rhizome, presumably to seal off healthy tissue with a physical barrier from continued pest attack. It is concluded that MJ induces polyphenolics in susceptible Mbwazirume banana that reduced C. sordidus damage.

中文翻译:

香蕉对大球藻取食和茉莉酸甲酯施用的防御反应

每年,由于撒哈拉以南非洲生长地区的香蕉象鼻虫(Cosmopolites sordidus)造成的根茎破坏,导致香蕉和大蕉产量损失25-75%。然而,根茎组织对C. sordidus侵袭的特定植物防御反应尚不清楚。因此,在这项研究中,我们评估了根状茎中的植物防御物质是否与幼虫损害程度相关,以及茉莉酸甲酯(MJ)的施用是否引起了更大的诱导抗弓形虫的植物防御能力。此外,我们试图通过组织化学染色揭示对根饲草食动物的响应的细胞修饰。对香蕉具有耐受性和敏感性的“ Km5”和“ Mbwazirume”香蕉品种C. sordidus,分别在盆栽试验用于评估百分比根茎损伤,叶片的叶绿素含量,总酚含量(TPC),响应于抗氧化能力和细胞形态C. sordidus攻击和/或MJ应用与未处理的对照植物。我们发现C. sordidus易感品种的根茎损伤为30%,而耐性品种的根茎损伤小于5%。根茎损伤百分率与叶片叶绿素含量无关,但与TPC和抗氧化能力均呈显着负线性关系。喂食幼虫在Km5中引起的多酚防御化合物的增加比在Mbwazirume中大得多。但是,这种反应在MJ处理中是相反的,这表明植物激素诱导易感植物对防御化学物质的合成进行了更多投资,从而降低了弓形虫的危害。组织染色表明木质素在更大的沉积和木栓质C. sordidus挑战根茎,大概是通过阻止有害生物持续发作的物理屏障来封闭健康组织。结论是,MJ在易感的Mbwazirume香蕉中诱导了多酚类物质,从而降低了C. sordidus的伤害。
更新日期:2020-08-26
down
wechat
bug