Bsoft offers many tools for the processing of tomographic tilt series and the interpretation of tomograms. Since I introduced tomography into Bsoft almost two decades ago, the field has advanced significantly, requiring refinement of old algorithms and development of new ones. The current direct detectors allow us to collect data more efficiently and with better quality, progressing towards automation. The goal is then to also automate alignment of tilt series and reconstruction. I added an estimation of the specimen thickness as well as fiducialless alignment, to augment the existing fiducial‐based alignment. High‐resolution work requires correction for the contrast transfer function, in tomography complicated by the tilted specimen. For this, I developed a method to generate a power spectrum using the whole micrograph, compensating for tilting. This is followed by routine determination of the contrast transfer function, and correction for it during reconstruction. The next steps involve interpretation of the tomogram, either by subtomogram averaging where possible, or by segmentation and modeling otherwise. Such interpretation actually constitutes the main time‐consuming part of tomography and is less amenable to automation compared to the initial reconstruction.

中文翻译：

---

Bsoft 中的高分辨率电子断层扫描和分段建模解释。

Bsoft 提供了许多工具来处理断层倾斜系列和断层图像的解释。自从我在大约 20 年前将断层扫描引入 Bsoft 以来，该领域取得了显着进步，需要改进旧算法并开发新算法。当前的直接检测器使我们能够更高效、更优质地收集数据，并朝着自动化方向发展。目标也是自动对齐倾斜系列和重建。我添加了对样本厚度的估计以及无基准对齐，以增强现有的基于基准的对齐。高分辨率工作需要对对比度传递函数进行校正，在由倾斜样品复杂化的断层扫描中。为此，我开发了一种使用整个显微照片生成功率谱的方法，以补偿倾斜。随后是对比度传递函数的常规确定，并在重建期间对其进行校正。接下来的步骤涉及对断层图的解释，在可能的情况下通过亚断层图平均，或者通过其他方式进行分割和建模。这种解释实际上构成了断层扫描的主要耗时部分，与初始重建相比不太适合自动化。