Clindamycin is used for infections caused by Gram‐positive and Gram‐negative anaerobic pathogens and Gram‐positive aerobes. Propionibacterium acnes is an important opportunistic microorganism of the human skin and is related to prostatitis. An LC–electrospray ionization–quadrupole time‐of‐flight–MS method was validated for determining clindamycin concentrations in plasma and prostate microdialysate. Clindamycin separation was carried out on a C₁₈ column at 0.5 mL/min. The mobile phase employed gradient elution of formic acid and methanol. A mass spectrometer was operated in positive electrospray ionization mode to monitor ion 425.1784 and 253.1152 for clindamycin and cimetidine (internal standard), respectively. Linearity was obtained at 0.5–10.0 μg/mL (plasma) and 0.05–1.0 μg/mL (microdialysate) with coefficients of determination ≥0.999. The intra‐ and inter‐day precision (coefficient of variation ‐ CV%) values were ≤13.83% and 12.51% for plasma, respectively, and ≤10.90% and 9.35% for microdialysate, respectively. The accuracy was between 90.82% and 108.25% for plasma, and 96.97% and 106.98% for microdialysate. The present method was fully validated and applied to investigate clindamycin concentrations in both plasma and prostate by microdialysis in Wistar rats (80 mg/kg, intravenous). Because the penetration of antibiotics into the prostate may be restricted, this method allows us to investigate the prostate concentrations of clindamycin for the first time.

中文翻译：

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LC-ESI-QTOF-MS方法在评估大鼠血浆和前列腺微透析液中克林霉素浓度中的应用。

克林霉素用于革兰氏阳性和革兰氏阴性厌氧性病原体以及革兰氏阳性需氧菌引起的感染。痤疮丙酸杆菌是人皮肤的重要机会微生物，与前列腺炎有关。液相色谱-电喷雾电离-四极杆飞行时间-质谱法已用于测定血浆和前列腺微透析液中克林霉素的浓度。克林霉素的分离在C _18上进行柱速度为0.5 mL / min。流动相采用甲酸和甲醇的梯度洗脱。质谱仪在正电喷雾电离模式下运行，分别监测离子425.1784和253.1152的克林霉素和西咪替丁（内标）。线性在0.5–10.0μg/ mL（血浆）和0.05–1.0μg/ mL（微量透析液）下获得，测定系数≥0.999。血浆中日间和日间精度（变异系数-CV％）值分别为≤13.83％和12.51％，微量透析液分别为≤10.90％和9.35％。血浆的准确度在90.82％到108.25％之间，微量透析液的准确度在96.97％和106.98％之间。本方法已得到充分验证，并用于通过微透析Wistar大鼠（80 mg / kg，静脉内）研究血浆和前列腺中的克林霉素浓度。