Cognitive impairments and poor performance on tasks needing behavioral flexibility are observable in chronic alcohol exposure. NeuroAid decreases cognitive deficits and improves functional outcomes by restoring neuronal circuits. The aim of the current study was to assess the hypothesis that ethanol exposure would induce neurobehavioral defects which may be reversed by the neuroprotective property of NeuroAid. Adult male Wistar rats were treated with saline, ethanol (0.2 g/kg), NeuroAid (0.8 g/kg) and ethanol (0.2 g/kg) + NeuroAid (0.8 g/kg). Then, behavioral tests were performed using the Y-maze apparatus, hot-plate and tail-flick apparatuses, locomotion apparatus as well as the loss of righting reflex (LORR) and hanging protocols (performance in a wire hanging test). Our results indicated that intraperitoneal (i.p.) administration of ethanol alone and administration of ethanol along with NeuroAid for one week reversed ethanol-induced spatial memory deficits in rats (P < 0.01). Interestingly, treatment with ethanol (0.2 g/kg) for one week induced nociception (P < 0.01). Moreover, one week administration of ethanol (0.2 g/kg) along with NeuroAid (0.8 g/kg) increased latency to LORR (P < 0.001) while four weeks administration of ethanol (0.2 g/kg) along with NeuroAid (0.8 g/kg) decreased sleep time (P < 0.01). In addition, a single administration of all drugs did not alter locomotor activity (P > 0.05) and hanging (P > 0.05). Improvement of behavioral tasks after one-week i.p. administration of ethanol and/or NeuroAid in comparison with a single administration of ethanol and/or NeuroAid may be due to the neuroprotective property of ethanol and/or NeuroAiD.

在慢性酒精暴露中可以观察到认知障碍和需要行为灵活性的任务的表现不佳。NeuroAid 通过恢复神经元回路减少认知缺陷并改善功能结果。本研究的目的是评估乙醇暴露会诱发神经行为缺陷的假设，而神经行为缺陷可能会被 NeuroAid 的神经保护特性逆转。成年雄性 Wistar 大鼠用盐水、乙醇 (0.2 g/kg)、NeuroAid (0.8 g/kg) 和乙醇 (0.2 g/kg) + NeuroAid (0.8 g/kg) 处理。然后，使用 Y 迷宫装置、热板和甩尾装置、运动装置以及翻正反射丧失 (LORR) 和悬挂协议（在线悬挂测试中的性能）进行行为测试。我们的结果表明，腹腔内（ipP  < 0.01)。有趣的是，用乙醇 (0.2 g/kg) 治疗 1 周会诱发伤害感受 (P < 0.01)。此外，乙醇 (0.2 g/kg) 和 NeuroAid (0.8 g/kg) 一起给药 1 周增加了 LORR ( P  < 0.001) 的潜伏期，而乙醇 (0.2 g/kg) 和 NeuroAid (0.8 g/ kg) 减少睡眠时间 ( P  < 0.01)。此外，所有药物的单次给药不会改变运动活动（P  > 0.05）和悬挂（ P > 0.05）。与乙醇和/或 NeuroAid 的单次给药相比，乙醇和/或 NeuroAid 一周 ip 给药后行为任务的改善可能是由于乙醇和/或 NeuroAiD 的神经保护特性。