Immunocompromised patients encounter fungal infections more frequently than healthy individuals. Conventional drugs associated health risk and resistance, portrayed fungal infections as a global health problem. This issue needs to be answered immediately by designing a novel anti-fungal therapeutic agent. Phytoactive molecules based therapeutics are most suitable candidate due to their low cytotoxicity and minimal side effects to the host. In this study, cinnamaldehyde (CA), an FDA approved phytoactive molecule present in cinnamon essential oil was incorporated into gellan (GA)/poly vinyl alcohol (PVA) based electrospun nanofibers to resolve the issues like low water solubility, high volatility and irritant effect associated with CA and also to enhance its therapeutic applications. The drug encapsulation, morphology and physical properties of the synthesized CA nanofibers were evaluated by FESEM, AFM, TGA, FTIR and static water contact angle analysis. The average diameters of CA encapsulated GA/PVA nanofibers and GA/PVA nanofibers were recorded to be 278.5 ± 57.8 nm and 204.03 ± 39.14 nm, respectively. These nanofibers were evaluated for their anti-biofilm activity against Candida using XTT (2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl]-2H-tetrazolium salt) reduction assay. Data demonstrated that CA encapsulated GA/PVA nanofibers can effectively eradicate 89.29% and 50.45% of Candida glabrata and Candida albicans biofilm respectively. CA encapsulated nanofibers exhibited brilliant antimicrobial property against Staphylococcus aureus and Pseudomonas aeruginosa. The cytotoxicity assay demonstrated that nanofibers loaded with CA have anticancer properties as it reduces cell viability of breast cancer cells (MCF-7) by 27.7%. These CA loaded GA/PVA (CA-GA/PVA) nanofibers could be used as novel wound dressing material and coatings on biomedical implants to eradicate biofilm.

免疫功能低下的患者比健康个体更常发生真菌感染。常规药物与健康风险和抵抗力相关，将真菌感染描述为全球性健康问题。需要通过设计新型抗真菌治疗剂立即解决此问题。基于植物活性分子的治疗剂因其低细胞毒性和对宿主的最小副作用而成为最合适的候选药物。在这项研究中，肉桂精油中存在的FDA批准的植物活性分子肉桂醛（CA）被掺入了基于结冷胶（GA）/聚乙烯醇（PVA）的电纺纳米纤维中，以解决诸如低水溶性，高挥发性和刺激性的问题与CA相关联，还可以增强其治疗应用。药物封装 通过FESEM，AFM，TGA，FTIR和静态水接触角分析评价了合成的CA纳米纤维的形貌和物理性能。CA封装的GA / PVA纳米纤维和GA / PVA纳米纤维的平均直径分别记录为278.5±57.8 nm和204.03±39.14 nm。评估了这些纳米纤维的抗生物膜活性假丝酵母使用XTT（2，3-双（2-甲氧基-4-硝基-5-磺基苯基）-5-[（苯氨基）-羰基] -2H-四唑鎓盐）还原测定。数据表明，CA包裹的GA / PVA纳米纤维可分别有效消灭光滑念珠菌和白色念珠菌生物膜的89.29％和50.45％。CA包裹的纳米纤维对金黄色葡萄球菌和铜绿假单胞菌具有出色的抗菌性能。细胞毒性试验表明，负载有CA的纳米纤维具有抗癌特性，因为它可使乳腺癌细胞（MCF-7）的细胞生存力降低27.7％。这些CA负载的GA / PVA（CA-GA / PVA）纳米纤维可用作新型伤口敷料和生物医学植入物上的涂层，以根除生物膜。