Residual contamination of water with MPH represents a severe environmental issue because it can affect non-target animals. Here we describe the behavioral effects in zebrafish after chronic contamination of water containing residues of MPH (0.1875, 1.875 and 3 ug/L). These doses are environmentally relevant since they reflect those found in wastewaters. We evaluated the behavioral effect through the novel tank test (NTT) and social preference test (SPT), and after euthanasia we analyzed oxidative stress parameters. Zebrafish exposed to MPH presented a social impairment, avoiding the conspecifics segment in the social preference test. In addition, MPH in the lowest concentration provoked an anxiolytic effect in the novel tank test. Oxidative stress is not related to these changes. Since the maintenance of an intact behavioral repertoire is crucial for species survival and fitness, our results demonstrate that residual contamination of water by MPH can be a threat to zebrafish, impacting directly to its well-being and survival in the aquatic environment.

MPH对水的残留污染是一个严重的环境问题，因为它会影响非目标动物。在这里，我们描述了含有MPH残留物（0.1875、1.875和3 ug / L）的慢性污染后在斑马鱼中的行为影响。这些剂量与环境有关，因为它们反映了废水中的剂量。我们通过新型的坦克测试（NTT）和社会偏好测试（SPT）评估了行为效果，安乐死后我们分析了氧化应激参数。暴露于MPH的斑马鱼出现了社交障碍，避免了社交偏好测试中的同种异体。另外，在新的储罐试验中，最低浓度的MPH引起了抗焦虑作用。氧化应激与这些变化无关。