Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants. However, cellulose in plants is accompanied by other polymers like hemicellulose, lignin, and pectin. On the other hand, pure cellulose can be produced by some microorganisms, with the most active producer being Acetobacter xylinum. A. senengalensis is a gram-negative, obligate aerobic, motile coccus, isolated from Mango fruits in Senegal, capable of utilizing a variety of sugars and produce cellulose. Besides, the production is also influenced by other culture conditions. Previously, we isolated and identified A. senengalensis MA1, and characterized the bacterial cellulose (BC) produced. The maximum cellulose production by A. senengalensis MA1 was pre-optimized for different parameters like carbon, nitrogen, precursor, polymer additive, pH, temperature, inoculum concentration, and incubation time. Further, the pre-optimized parameters were pooled, and the best combination was analyzed by using Central Composite Design (CCD) of Response Surface Methodology (RSM). Maximum BC production was achieved with glycerol, yeast extract, and PEG 6000 as the best carbon and nitrogen sources, and polymer additive, respectively, at 4.5 pH and an incubation temperature of 33.5 °C. Around 20% of inoculum concentration gave a high yield after 30 days of inoculation. The interactions between culture conditions optimized by CCD included alterations in the composition of the HS medium with 50 mL L− 1 of glycerol, 7.50 g L− 1 of yeast extract at pH 6.0 by incubating at a temperature of 33.5 °C along with 7.76 g L− 1 of PEG 6000. This gave a BC yield of wet weight as 469.83 g L− 1. The optimized conditions of growth medium resulted in enhanced production of bacterial cellulose by A. senegalensis MA1, which is around 20 times higher than that produced using an unoptimized HS medium. Further, the cellulose produced can be used in food and pharmaceuticals, for producing high-quality paper, wound dressing material, and nanocomposite films for food packaging.

中文翻译：

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塞内加尔醋杆菌MA1生产细菌纤维素的最佳培养条件。

纤维素是地球上用途最广泛的生物分子，可以从植物中大量获得。但是，植物中的纤维素还伴有其他聚合物，例如半纤维素，木质素和果胶。另一方面，纯纤维素可以由某些微生物产生，最活跃的产生者是木醋杆菌。senengalensis是一种革兰氏阴性的专性好氧运动球菌，从塞内加尔的芒果果实中分离出来，能够利用多种糖类并产生纤维素。此外，生产还受到其他培养条件的影响。以前，我们分离并鉴定了A. senengalensis MA1，并鉴定了产生的细菌纤维素（BC）。针对不同的参数（例如碳，氮，前体，聚合物添加剂，pH，温度，接种浓度和孵育时间。此外，汇总了预先优化的参数，并使用响应表面方法学（RSM）的中央复合设计（CCD）分析了最佳组合。使用甘油，酵母提取物和PEG 6000作为最佳碳和氮源，以及聚合物添加剂，分别在4.5 pH和33.5°C的孵育温度下实现了最大的BC产量。接种30天后，约20％的接种物浓度可获得高产量。通过CCD优化的培养条件之间的相互作用包括：通过在33.5°C和7.76 g的温度下孵育，改变pH值为6.0的50 mL L-1甘油，7.50 g L-1酵母提取物的HS培养基的组成PEG 6000的L-1。得到的湿重BC产量为469.83 g L-1。优化的生长培养基条件可以提高塞内加尔农杆菌MA1的细菌纤维素产量，这比使用未优化的HS培养基高出约20倍。此外，产生的纤维素可用于食品和药品中，用于生产高质量的纸，伤口敷料和用于食品包装的纳米复合膜。