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Unveiling Cells' Local Environment during Cryopreservation by Correlative In Situ Spatial and Thermal Analyses.
The Journal of Physical Chemistry Letters ( IF 5.7 ) Pub Date : 2020-08-25 , DOI: 10.1021/acs.jpclett.0c01729
Kankan Qin 1 , Corentin Eschenbrenner 1 , Felix Ginot 2 , Dmytro Dedovets 2 , Thibaud Coradin 1 , Sylvain Deville 2, 3 , Francisco M Fernandes 1
Affiliation  

Cryopreservation is the only fully established procedure to extend the lifespan of living cells and tissues, a key to activities spanning from fundamental biology to clinical practice. Despite its prevalence and impact, the central aspects of cryopreservation, such as the cell’s physicochemical environment during freezing, remain elusive. Here we address that question by coupling in situ microscopic directional freezing to visualize cells and their surroundings during freezing with the freezing-medium phase diagram. We extract the freezing-medium spatial distribution in cryopreservation, providing a tool to describe the cell vicinity at any point during freezing. We show that two major events define the cells’ local environment over time: the interaction with the moving ice front and the interaction with the vitreous moving front, a term we introduce here. Our correlative strategy may be applied to cells relevant to clinical research and practice and may help in the design of new cryoprotective media based on local physicochemical cues.

中文翻译:

通过相关的原位空间和热分析揭示冷冻保存过程中细胞的局部环境。

冷冻保存是延长活细胞和组织寿命的唯一完全成熟的程序,这是从基础生物学到临床实践活动的关键。尽管它具有普遍性和影响力,但冷冻保存的主要方面(如细胞在冷冻过程中的物理化学环境)仍然难以捉摸。在这里,我们通过原位耦合解决这个问题显微镜定向冷冻,以冷冻介质相图观察冷冻过程中的细胞及其周围环境。我们提取了冷冻保存中冷冻介质的空间分布,提供了一种工具来描述冷冻过程中任何时候的细胞附近。我们展示了随着时间的流逝,两个主要事件定义了细胞的局部环境:与移动的冰锋的相互作用和与玻璃的移动的锋锋的相互作用,我们在此引入一个术语。我们的相关策略可能会应用于与临床研究和实践相关的细胞,并可能有助于基于局部理化线索设计新的冷冻保护培养基。
更新日期:2020-09-18
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