Flavonoids are important active components of traditional Chinese medicines (TCMs) because of their many biological activities. We studied the interaction between 17 polysaccharides and eight flavonoids via high-performance liquid chromatography (HPLC) and the effect of the interactions on the solubility and stability of the flavonoids. The effect of the polysaccharides on the solubility of flavonoids was analyzed by statistical methods and showed significant solubility improvements. The constant temperature acceleration method (90°C/pH 9 buffer solution) was used to measure the degradation kinetics and half-life of flavonoids with and without polysaccharides. All the polysaccharides displayed a stabilizing effect on all eight flavonoids. The stabilizing effects varied in the order: quercetin, baicalein > baicalin > galuteolin > daidzin > rutin > luteolin > daidzien. The phase-solubility method was applied to quercetin and baicalein to study the mechanism of action of the polysaccharides. It appeared that the two flavonoids could form 1 : 1 inclusion complexes with polysaccharides, which may be one of the factors increasing solubility and stability. These findings increase our understanding of the role of endogenous polysaccharides in TCM in improving the stability and bioavailability of bioactive flavonoids.

中文翻译：

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17种中草药多糖对8种黄酮类化合物的增溶稳定作用研究

黄酮类化合物具有多种生物活性，是中药的重要活性成分。我们通过高效液相色谱 (HPLC) 研究了 17 种多糖和 8 种黄酮类化合物之间的相互作用以及相互作用对黄酮类化合物的溶解度和稳定性的影响。通过统计方法分析了多糖对黄酮类化合物溶解度的影响，并显示出显着的溶解度改进。恒温加速法（90°C/pH 9 缓冲溶液）用于测量含和不含多糖的黄酮类化合物的降解动力学和半衰期。所有的多糖对所有八种黄酮类化合物都表现出稳定作用。稳定作用依次为：槲皮素、黄芩素 > 黄芩素 > galuteolin > daidzin > 芦丁 > 木犀草素 > daidzien。将相溶度法应用于槲皮素和黄芩素，研究多糖的作用机制。看来这两种黄酮类化合物可以与多糖形成1:1的包合物，这可能是增加溶解度和稳定性的因素之一。这些发现增加了我们对中药中内源性多糖在提高生物活性黄酮类化合物的稳定性和生物利用度方面的作用的理解。