Abstract

Background

Studies have just started delineating the role of Toll-like receptors (TLRs) in the pathogenesis of multiple sclerosis (MS).

Objectives

To investigate the expression of TLR-2 and TLR-4 on peripheral blood neutrophils and lymphocytes in Egyptian patients with MS, and to examine the role of TLR-2 and TLR-4 expression as a candidate biomarker for MS diagnosis.

Methods

A total of 84 patients with newly diagnosed MS and 68 healthy controls were included in this study. The expression levels of TLR-2 and TLR-4 were assessed by flow cytometry technique using appropriate monoclonal antibodies.

Results

TLR-2 demonstrated a significantly higher expression on the lymphocytes and neutrophils of patients, whereas that of TLR-4 was significantly higher only on lymphocytes than those in the control group. However, there was no significant difference between patients with relapsing remitting MS and those with secondary progressive MS in terms of TLR-2 and TLR-4 expression. The expression of TLR-2 and TLR-4 on the lymphocytes and neutrophils of patients showed no significant correlation with either the duration of the disease or disability. The sensitivity and specificity of TLR-2 expression on lymphocytes and neutrophils to diagnose MS were 73.81%, 70.59%, 69.05%, and 52.94%, respectively. The sensitivity and specificity of TLR-4 expression on lymphocytes and neutrophils were 88.10%, 79.41%, 78.57%, and 76.47%, respectively.

Conclusion

The expression of TLR-2 and TLR-4 on peripheral blood neutrophils and lymphocytes has a potential role in the pathogenesis of MS. TLR-4 expression on lymphocytes and neutrophils could be used as a potential biomarker to diagnose MS.

中文翻译：

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埃及多发性硬化症患者外周血淋巴细胞和中性粒细胞Toll样受体2和4的表达

摘要

背景

研究刚刚开始描述 Toll 样受体 (TLR) 在多发性硬化症 (MS) 发病机制中的作用。

目标

研究埃及 MS 患者外周血中性粒细胞和淋巴细胞中 TLR-2 和 TLR-4 的表达，并检查 TLR-2 和 TLR-4 表达作为 MS 诊断候选生物标志物的作用。

方法

本研究共纳入 84 名新诊断 MS 患者和 68 名健康对照者。使用适当的单克隆抗体通过流式细胞术技术评估 TLR-2 和 TLR-4 的表达水平。

结果

TLR-2在患者淋巴细胞和中性粒细胞上的表达显着升高，而TLR-4仅在淋巴细胞上显着高于对照组。然而，在 TLR-2 和 TLR-4 表达方面，复发缓解型 MS 患者与继发进展型 MS 患者之间没有显着差异。患者淋巴细胞和中性粒细胞上TLR-2和TLR-4的表达与疾病持续时间或残疾无显着相关性。淋巴细胞和中性粒细胞TLR-2表达诊断MS的敏感性和特异性分别为73.81%、70.59%、69.05%和52.94%。TLR-4表达对淋巴细胞和中性粒细胞的敏感性和特异性分别为88.10%、79.41%、78.57%和76.47%。

结论

外周血中性粒细胞和淋巴细胞上TLR-2和TLR-4的表达在MS的发病机制中具有潜在作用。淋巴细胞和中性粒细胞上的 TLR-4 表达可用作诊断 MS 的潜在生物标志物。