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Dietary supplementation of host‐associated lactic acid bacteria modulates growth, metabolic activities, and immune‐related gene expression in giant freshwater prawn, Macrobrachium rosenbergii
Journal of the World Aquaculture Society ( IF 2.8 ) Pub Date : 2020-08-26 , DOI: 10.1111/jwas.12734
Md. Abdul Kader 1 , Nursyafiqah Zahidah Azahar 1 , Shumpei Iehata 1 , Mahbuba Bulbul 2 , Md. Moudud Islam 3 , Joyshri Sarker 3 , Mariom 4 , Md. Moshiur Rahman 5 , Md Asaduzzaman 3
Affiliation  

The present study was carried out to evaluate the dietary effects of host‐associated lactic acid bacteria on growth performance, metabolic enzyme activities, and immune response of Macrobrachium rosenbergii juveniles. To formulate the test diets, a control (Con) diet was supplemented with a commercial probiotic and three host‐derived bacteria Enterococcus faecalis (EC), Lactococcus lactis I (LC‐I), and L. lactis II (LC‐II), which were previously isolated from the gastrointestinal tract of adult individuals of M. rosenbergii. Juvenile M. rosenbergii (0.65 ± 0.008 g) were randomly stocked at 20 individuals/100 L of fiberglass tanks with three replications for each test diet. After 50 days, juveniles fed the diets LC‐I and LC‐II showed significantly higher (p < .05) weight gain, specific growth rate, and the lowest feed conversion ratio. The analyses of glutamic oxaloacetate transaminase and glutamic pyruvate transaminase in muscle and hepatopancreas revealed significantly (p < .05) reduced values in LC‐I fed juveniles. The total hemocyte count and phenoloxidase activity were significantly increased (p < .05) in LC‐I and LC‐II fed juveniles. The relative mRNA expression patterns of immune‐related α2‐M, LGBP, ProPO, Cu, Zn‐SOD, TG, PE, AKP, and ACP genes were significantly (p < .05) upregulated in juveniles fed with LC‐I followed by the diet LC‐II. Finally, the study suggests that the growth performance and immune response of M. rosenbergii can be improved through supplementation of host‐associated L. lactis bacteria for its higher production.

中文翻译:

膳食补充宿主相关的乳酸菌可调节淡水虾罗氏沼虾的生长,代谢活性和免疫相关基因表达

本研究旨在评估宿主相关乳酸菌对罗氏沼虾幼体生长性能,代谢酶活性和免疫反应的饮食影响。为了配制测试饮食,在对照(Con)饮食中添加了商业益生菌和三种宿主来源的细菌粪便肠球菌(EC),乳酸乳球菌I(LC-I)和乳酸乳球菌II(LC-II),这些以前是从罗氏沼虾的成年个体的胃肠道中分离出来的。罗氏沼虾少年(0.65±0.008 g)以20个人/ 100 L的玻璃纤维罐随机存放,每种测试饮食重复三次。50天后,喂食LC-1和LC-2型饲料的幼鱼 体重增加,比生长率和最低饲料转化率显着提高(p <.05)。对肌肉和肝胰腺中的谷氨酸草酰乙酸转氨酶和谷氨酸丙酮酸转氨酶的分析表明, 喂食LC-1的幼鱼的值显着降低(p <.05)。 LC-I和LC-II喂养的少年的总血细胞计数和酚氧化酶活性显着增加(p <.05)。免疫相关α2-M,LGBP,ProPO,Cu,Zn-SOD,TG,PE,AKP和ACP基因的相对mRNA表达模式显着(p <.05)在饲喂LC-I和日粮LC-II的少年中上调。最后,该研究表明,通过补充宿主相关的乳酸乳球菌可以提高罗氏沼虾的生长性能和免疫反应,从而提高产量。
更新日期:2020-08-26
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