The effects of food type, salinity and temperature on the growth performance and antioxidant status of Hippocampus reidi juveniles at 2.5 months old were evaluated in this study. Three trials were conducted for 56 days. Seahorses fed frozen mysid shrimp (MD) exhibited significantly higher survival, growth performance and feed efficiency compared with that of seahorses fed frozen adult Artemia (AD) or a mixture consisting of MD and AD at the ratio of 1:1 (MAD). Mortality of seahorse cultured at 30°C reached 100% at 49 days. Despite no significant difference, the survival and growth performance of seahorses were higher at 26 than 28°C. Seahorses had better growth performance at salinities of 15–20 ppt, but the survival and growth performance of seahorses were not significantly different among salinity groups. Seahorses fed MD or MAD had better antioxidant status based on the results of total phenolic content (TPC) and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activity in the MD and MAD groups, and significantly higher reducing power in MD than AD. No significant difference in all antioxidant indicators was recorded in 26 and 28°C groups. TPC, DPPH radical scavenging activity and ferric reducing antioxidant power activity of seahorses were significantly higher at 15 and 20 ppt, and metal chelating activity of seahorses at 15 ppt was significantly higher than that of seahorses at higher salinities. Based on these results, H. reidi juvenile is recommended to be fed MD at a temperature of 26°C and salinity of 15–20 ppt.

中文翻译：

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食物类型，温度和盐度对长鼻海马海马生长性能和抗氧化状态的影响

本研究评估了食物类型，盐度和温度对2.5个月大的海马幼体生长性能和抗氧化状态的影响。三项试验进行了56天。与饲喂冷冻成年卤虫的海马相比，饲喂冷冻Mysid虾（MD）的海马表现出更高的存活率，生长性能和饲料效率（AD）或MD和AD以1：1的比例组成的混合物（MAD）。在30天培养的海马的死亡率在49天时达到100％。尽管没有显着差异，但在26°C时海马的存活和生长性能要高于28°C。在盐度为15–20 ppt时，海马的生长性能更好，但是盐度组之间海马的存活和生长性能没有显着差异。根据MD和MAD组的总酚含量（TPC）和2,2-二苯-1--1-吡啶并肼基（DPPH）清除自由基的结果，饲喂MD或MAD的海马具有更好的抗氧化状态，并且MD的还原力明显更高比公元。在26和28°C组中，所有抗氧化剂指标均无显着差异。TPC，在较高盐度下，海马的DPPH自由基清除活性和三价铁还原抗氧化能力活性在15和20 ppt时显着较高，在15 ppt时，海马的金属螯合活性显着高于海马。根据这些结果，建议在温度为26°C，盐度为15–20 ppt的条件下喂食H. reidi幼鱼。