Prenatal exposure during the embryonic period has positive or adverse effect on newborn brain development. Neuroprotective activity of the hesperidin is well documented but there is no evidence for maternal exposure to hesperidin on offspring reflexive motor behaviors. So, the aim of the current study was to determine the prenatal exposure to hesperidin on reflexive motor behaviors in mice offspring. Forty pregnant female NMRI mice (8–10 weeks old) were allocated into four groups. Group 1 kept as control and groups 2–4 intraperitoneal (i.p) injected with hesperidin (0.1, 0.5, and 1 mg/kg) on days of 5, 8, 11, 14, and 17 of pregnancy. The control group injected with saline at the same days. Following delivery, 20 pups from each litter were selected and reflexive motor behaviors determined using ambulation, hind‐limb foot angle, surface righting, hind‐limb strength, grip strength, front‐limb suspension, and negative geotaxis tests. At the end of the study serum Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione peroxidase (GPx), and total antioxidant status (TAS) levels were determined. According to the results, maternal exposure to hesperidin (0.1, 0.5, and 1 mg/kg) increased ambulation score, front‐limb suspension time, and hind‐limb suspension score in mice offspring compared to the control group (p < .05). Hesperidin (0.1, 0.5, and 1 mg/kg) decreased hind‐limb foot angle in mice offspring compared to the control group (p < .05). Prenatal exposure to hesperidin (0.5 and 1 mg/kg) significantly increased the surface righting and grip strength in comparison to the control group (p < .05). Hesperidin (0.1, 0.5, and 1 mg/kg) decreased MDA and increased SOD and GPx levels in mice offspring (p < .05). These results suggested hesperidin exposure during pregnancy has positive effect on reflexive motor behaviors in mice offspring may be due to its antioxidant activity.

中文翻译：

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产前暴露于橙皮苷可改善小鼠后代的反射性运动行为

胚胎期的产前暴露对新生儿大脑发育有正面或负面影响。橙皮苷的神经保护活性有据可查，但没有证据表明母体暴露于橙皮苷会影响后代的反射性运动行为。因此，本研究的目的是确定产前暴露于橙皮苷对小鼠后代反射性运动行为的影响。40 只怀孕的雌性 NMRI 小鼠（8-10 周大）被分成四组。第 1 组作为对照组，第 2-4 组在怀孕第 5、8、11、14 和 17 天腹膜内 (ip) 注射橙皮苷（0.1、0.5 和 1 mg/kg）。对照组在同一天注射生理盐水。分娩后，从每窝中选择 20 只幼崽，并使用步行、后肢足角、表面扶正、后肢力量、握力、前肢悬吊和负地势测试。在研究结束时，测定血清丙二醛 (MDA)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GPx) 和总抗氧化状态 (TAS) 水平。根据结果​​，与对照组相比，母体暴露于橙皮苷（0.1、0.5 和 1 mg/kg）可增加小鼠后代的行走评分、前肢悬吊时间和后肢悬吊评分（p < .05） . 与对照组相比，橙皮苷（0.1、0.5 和 1 mg/kg）降低了小鼠后代的后肢足角（p < .05）。与对照组相比，产前暴露于橙皮苷（0.5 和 1 毫克/千克）显着增加了表面翻正和握力（p < .05）。橙皮苷 (0.1, 0.5, 和 1 mg/kg) 降低小鼠后代的 MDA 并增加 SOD 和 GPx 水平（p < .05）。这些结果表明，怀孕期间橙皮苷暴露对小鼠后代的反射性运动行为有积极影响，可能是由于其抗氧化活性。