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The anti‐inflammatory properties of Rhododendron molle G. Don leaf extract in LPS‐induced RAW 264.7
Chemistry & Biodiversity ( IF 2.9 ) Pub Date : 2020-09-21 , DOI: 10.1002/cbdv.202000477
Luye Zong 1 , Jin Zhang 1 , Liangfang Dai 1 , Jian Liu 1 , Yan Yang 1 , Jiankun Xie 1 , Xiangdong Luo 1
Affiliation  

Rhododendron molle G.Don is a well‐known traditional medicine which has been used to treat rheumatic inflammation. In this study, an inflammatory model of lipopolysaccharide (LPS)‐stimulated RAW264.7 cells was established to analyze the anti‐inflammatory effect and potential mechanism of the methanol extract of R. molle leaves (RLE). The production of NO and the expression of tumor necrosis factor by LPS were detected by Griess reaction and enzyme linked immunosorbent assay (ELISA). The mRNA expression of TNF‐α, IL‐1β, IL‐6, COX‐2 and iNOS was measured by qRT‐PCR assay. Griess and qRT‐PCR showed that the RLE could significantly concentration‐dependently inhibit NO production and the expression of many pro‐inflammatory cytokines and inflammatory‐related enzymes. Scanning electron microscope (SEM) analysis indicated that RLE could inhibit LPS‐stimulated RAW264.7 macrophages activation. The protein level of TNF‐α and IL‐1β were decreased over 50 % at 100 μg/ml of RLE, as detected by ELISA. These results indicated that RLE had strong anti‐inflammatory and immunomodulatory activity.

中文翻译:

Rhododendron molle G. Don 叶提取物在 LPS 诱导的 RAW 264.7 中的抗炎特性

Rhododendron molle G.Don 是一种著名的传统药物,已被用于治疗风湿性炎症。本研究建立了脂多糖 (LPS) 刺激的 RAW264.7 细胞的炎症模型,以分析紫荆叶甲醇提取物 (RLE) 的抗炎作用和潜在机制。通过Griess反应和酶联免疫吸附试验(ELISA)检测LPS对NO的产生和肿瘤坏死因子的表达。qRT-PCR 检测 TNF-α、IL-1β、IL-6、COX-2 和 iNOS 的 mRNA 表达。Griess 和 qRT-PCR 表明,RLE 可以显着浓度依赖性地抑制 NO 的产生以及许多促炎细胞因子和炎症相关酶的表达。扫描电子显微镜 (SEM) 分析表明,RLE 可以抑制 LPS 刺激的 RAW264.7 巨噬细胞活化。ELISA 检测到,当 RLE 为 100 μg/ml 时,TNF-α 和 IL-1β 的蛋白质水平降低了 50% 以上。这些结果表明 RLE 具有很强的抗炎和免疫调节活性。
更新日期:2020-09-21
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