A new approach to the quantitative analysis of aromatic metabolites in cerebrospinal fluid samples of neurosurgical patients based on microextraction by packed sorbent coupled with derivatization and GC–MS was developed. Analytical characteristics such as recoveries (40–90%), limit of detection (0.1–0.3 μm) and limit of quantitation (0.4–0.7 μm) values, accuracy (<±20%), precision (<20%) and linear correlations (R² ≥ 0.99) over a 0.4–10 μm range of concentrations demonstrated that microextraction by packed sorbent provides results for the quantitative analysis of target compounds comparable with those for liquid–liquid extraction. Similar results were achieved using 40 μl of sample for microextraction by packed sorbent instead of 200 μl for liquid–liquid extraction. Benzoic, 3‐phenylpropionic, 3‐phenyllactic, 4‐hydroxybenzoic, 2‐(4‐hydroxyphenyl)acetic, homovanillic and 3‐(4‐hydroxyphenyl)lactic acids were found in cerebrospinal fluid samples (n = 138) of neurosurgical patients in lower concentrations than in serum samples (n = 110) of critically ill patients. Analysis of the cerebrospinal fluid and serum samples taken at the same time from neurosurgical patients (n = 5) revealed similar results for patients without infection and multidirectional results for patients with central nervous system infection. Our preliminary results demonstrate the necessity of further evaluating the aromatic compound profile in cerebrospinal fluid for its subsequent verification for potential diagnostic markers.

中文翻译：

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吸附吸附剂微萃取-气相色谱-质谱法液-液萃取微萃取神经外科患者脑脊液中芳香族化合物的代谢谱

开发了一种新的定量分析神经外科患者脑脊液样品中芳香族代谢物的方法，该方法基于填充吸附剂与衍生化和GC-MS的微萃取。分析特性如回收率（40-90％），检测限（0.1-0.3μ米）和定量限（0.4-0.7μ米）的值，精度（<±20％），精密（<20％）和线性关系（- [R ² 在0.4-10μ≥0.99）米浓度范围表明，填充吸附剂的微萃取可提供与液-液萃取相当的目标化合物定量分析结果。使用40μl样品通过填充吸附剂进行微萃取而不是200μl进行液-液萃取，也获得了相似的结果。 在下层神经外科患者的脑脊液样本中发现苯甲酸，3-苯基丙酸，3-苯基乳酸，4-羟基苯甲酸，2-（4-羟基苯基）乙酸，高香草酸和3-（4-羟基苯基）乳酸（n = 138） 重症患者的血清浓度（n = 110）。同时分析神经外科患者的脑脊液和血清样本（n = 5）对于未感染的患者显示相似的结果，对于中枢神经系统感染的患者显示多方向的结果。我们的初步结果表明，有必要进一步评估脑脊液中的芳香族化合物概况，以用于随后的潜在诊断标志物验证。