We previously revealed adult reactive neurogenesis in deafferented vestibular nuclei following unilateral vestibular neurectomy (UVN) in the feline model. We recently replicated the same surgery in a rodent model and aimed to elucidate the origin and fate of newly generated cells following UVN. We used specific markers of cell proliferation, glial reaction, and cell differentiation in the medial vestibular nucleus (MVN) of adult rats. UVN induced an intense cell proliferation and glial reaction with an increase of GFAP-Immunoreactive (Ir), IBA1-Ir and Olig2-Ir cells 3 days after the lesion in the deafferented MVN. Most of the newly generated cells survived after UVN and differentiated into oligodendrocytes, astrocytes, microglial cells and GABAergic neurons. Interestingly, UVN induced a significant increase in a population of cells colocalizing SOX2 and GFAP 3 days after lesion in the deafferented MVN indicating the probable presence of multipotent cells in the vestibular nuclei. The concomitant increase in BrdU- and SOX2-Ir cells with the presence of SOX2 and GFAP colocalization 3 days after UVN in the deafferented MVN may support local mitotic activity of endemic quiescent neural stem cells in the parenchyma of vestibular nuclei.

我们之前在猫科动物模型中揭示了单侧前庭神经切除术 (UVN) 后传入的前庭神经核中的成人反应性神经发生。我们最近在啮齿动物模型中复制了相同的手术，旨在阐明 UVN 后新生成细胞的起源和命运。我们在成年大鼠的内侧前庭核 (MVN) 中使用了细胞增殖、神经胶质反应和细胞分化的特定标志物。UVN 诱导强烈的细胞增殖和神经胶质反应，GFAP 免疫反应性 (Ir)、IBA1-Ir 和 Olig2-Ir 细胞在传入神经阻滞的 MVN 损伤后 3 天增加。大多数新生成的细胞在 UVN 后存活并分化为少突胶质细胞、星形胶质细胞、小胶质细胞和 GABA 能神经元。有趣的是，UVN 在损伤后 3 天在去传入神经的 MVN 中诱导 SOX2 和 GFAP 共定位的细胞群显着增加，表明前庭核中可能存在专能细胞。在去传入神经的 MVN 中 UVN 后 3 天，随着 SOX2 和 GFAP 共定位的存在，BrdU-和 SOX2-Ir 细胞的伴随增加可能支持前庭核实质中地方性静止神经干细胞的局部有丝分裂活动。