Long-term hyperglycemia associated with diabetes mellitus (DM) causes damage to various organs and tissues, including the eyes, kidneys, heart, blood vessels and nerves. Rubus Suavissimus S. Lee (RS), a shrub whose leaves are used in traditional Chinese medicine (TCM), has been shown to exert hypoglycemic effects in DM patients. However, the underlying mechanism is unclear. This was investigated in the present study in a rat model of streptozotocin-induced type 1 diabetes mellitus (T1DM) by ¹ HNMR analysis. We identify 9 metabolites whose levels were altered in T1DM rats compared to control rats, namely, lactate, acetate, pyruvate, succinate, 2-oxoglutarate, citrate, creatinine, allantoin, and hippurate, which are mostly related to glycolysis/ gluconeogenesis, pyruvate metabolism, TCA cycle, and other metabolism. The observed pathologic changes in the levels of these metabolites in T1DM rats were reversed by treatment with RS. Thus, RS exerts effects in T1DM rats by regulating the three abnormal metabolic pathways synergistically. These findings provide supporting evidence for the therapeutic efficacy of this TCM formulation in the treatment of DM.

与糖尿病（DM）相关的长期高血糖症会损害包括眼睛，肾脏，心脏，血管和神经在内的各种器官和组织。已显示其叶子用于中药（TCM）的灌木Rubus Suavissimus S. Lee（RS）对DM患者具有降血糖作用。但是，其潜在机制尚不清楚。本研究在链脲佐菌素诱导的1型糖尿病（T1DM）的大鼠模型中研究了¹HNMR分析。我们确定9种代谢物在T1DM大鼠中的水平与对照大鼠相比发生了变化，即乳酸，乙酸盐，丙酮酸，琥珀酸，2-氧戊二酸，柠檬酸，肌酐，尿囊素和马尿酸，这些代谢物主要与糖酵解/糖异生，丙酮酸代谢有关，TCA循环等新陈代谢。通过RS治疗可以逆转T1DM大鼠中这些代谢产物水平的病理变化。因此，RS通过协同调节三种异常代谢途径在T1DM大鼠中发挥作用。这些发现为该中药制剂在DM治疗中的治疗功效提供了支持性证据。