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Characterization of motor function in mice developmentally exposed to ethanol using the Catwalk system: Comparison with the triple horizontal bar and rotarod tests.
Behavioural Brain Research ( IF 2.7 ) Pub Date : 2020-08-26 , DOI: 10.1016/j.bbr.2020.112885
Belkis Jacquez 1 , Hyesun Choi 1 , Clark W Bird 1 , David N Linsenbardt 1 , C Fernando Valenzuela 1
Affiliation  

Studies with human subjects indicate that ethanol exposure during fetal development causes long-lasting alterations in motor coordination that are, in part, a consequence of cerebellar damage. Studies with rats exposed to ethanol during the neonatal brain growth spurt have consistently recapitulated these deficits. However, studies with mice have yielded mixed results. We hypothesized that the use of highly sensitive motor function tests, such as the Catwalk test, would reliably detect motor function deficits in mice developmentally exposed to ethanol. Venus-vesicular GABA transporter transgenic mice were ethanol exposed during postnatal days 4–9 using vapor inhalation chambers and then subjected to the Catwalk test during adolescence. Catwalk data were rigorously analyzed using an innovative multistep statistical approach. For comparison, motor coordination and strength were assessed with the triple horizontal bar and rotarod tests. Unexpectedly, we found that out of 186 parameters analyzed in the Catwalk test, only one was affected by ethanol exposure (i.e., reduced coupling between left front paw and the right hind paw). In the triple horizontal bar test, ethanol-exposed mice were able to hold to the bars for less time than controls. Surprisingly, ethanol-exposed mice performed better in the rotarod test than controls. These data indicate that neonatal ethanol exposure of mice causes mixed effects on motor function during adolescence. The Catwalk test suggests that gait is generally preserved in these mice, whereas the triple horizontal bar test revealed deficits on motor strength and the rotarod test an increase in motor coordination.



中文翻译:

使用 Catwalk 系统对发育暴露于乙醇的小鼠的运动功能进行表征:与三重单杠和旋转杆测试的比较。

对人类受试者的研究表明,在胎儿发育期间接触乙醇会导致运动协调的长期改变,部分原因是小脑损伤。对在新生儿大脑生长突增期间暴露于乙醇的大鼠的研究一致地概括了这些缺陷。然而,对小鼠的研究产生了好坏参半的结果。我们假设使用高度敏感的运动功能测试,如时装表演测试,可以可靠地检测出发育暴露于乙醇的小鼠的运动功能缺陷。金星-囊泡 GABA 转运蛋白转基因小鼠在出生后第 4-9 天使用蒸气吸入室暴露于乙醇,然后在青春期进行猫步测试。使用创新的多步统计方法对时装表演数据进行了严格分析。为了比较,运动协调性和力量通过三重单杠和旋转杆测试进行评估。出乎意料的是,我们发现在 Catwalk 测试中分析的 186 个参数中,只有一个受到乙醇暴露的影响(即左前爪和右后爪之间的耦合减少)。在三重单杠测试中,与对照组相比,暴露于乙醇的小鼠能够握住横杠的时间更短。令人惊讶的是,乙醇暴露的小鼠在旋转棒测试中的表现优于对照组。这些数据表明,小鼠的新生儿乙醇暴露会对青春期的运动功能产生混合影响。Catwalk 测试表明这些小鼠的步态通常保持不变,而三重单杠测试显示运动强度存在缺陷,而旋转杆测试显示运动协调性增加。

更新日期:2020-09-02
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