当前位置:
X-MOL 学术
›
Hortic. Environ. Biotechnol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Enhanced detoxification of exogenous toluene gas in transgenic Ardisia pusilla expressing AtNDPK2 gene
Horticulture, Environment, and Biotechnology ( IF 2.4 ) Pub Date : 2020-08-26 , DOI: 10.1007/s13580-020-00275-1 Chang Ho Ahn , Nan-Sun Kim , Ju Young Shin , Young Ah Lee , Kwang Jin Kim , Jeong Ho Kim , Pil Man Park , Hye Ryun An , Yae-Jin Kim , Won Hee Kim , Su Young Lee
Horticulture, Environment, and Biotechnology ( IF 2.4 ) Pub Date : 2020-08-26 , DOI: 10.1007/s13580-020-00275-1 Chang Ho Ahn , Nan-Sun Kim , Ju Young Shin , Young Ah Lee , Kwang Jin Kim , Jeong Ho Kim , Pil Man Park , Hye Ryun An , Yae-Jin Kim , Won Hee Kim , Su Young Lee
The Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) gene is known to regulate the cellular redox state, and to enhance tolerance to multiple stressors in plants. In this study, we transferred AtNDPK2 under the stress-inducible promoter SWPA2 into Ardisia pusilla to enhance the plants’ ability to detoxify toluene gas. Thirty transgenic A. pusilla lines were confirmed by PCR analysis with AtNDPK2 and NPTII gene-specific primers. In addition, four transgenic A. pusilla lines were further confirmed by Southern blot analysis to verify the gene copy number. Three transgenic lines showed a single-copy transgene insertion, and one transgenic line had two transgene insertions. To test the gene expression of AtNDPK2 in the transgenic A. pusilla lines exposed to and not exposed to toluene treatment, qRT-PCR analysis was performed. The gene expression of AtNDPK2 in transgenic A. pusilla plants exposed to toluene treatment was significantly higher than that of transgenic plants not exposed to toluene treatment. Finally, we measured toluene removal efficiency of the transgenic and non-transgenic A. pusilla lines exposed to toluene-contaminated air. There was a statistically significant difference between the transgenic and non-transgenic A. pusilla lines at all time points (p < 0.001). The highest toluene removal efficiency (797.33 ± 59.41 µg m−3 cm−2 leaf area) was recorded in the transgenic A. pusilla line NDPK2-12-4 after 3 h of exposure to toluene, while the non-transgenic line showed little toluene removal efficiency (206.2 ± 31.19 µg m−3 cm−2 leaf area). These results suggest that the capacity for detoxifying toluene gas is related to the AtNDPK2 gene in A. pusilla. Therefore, this study provides useful results to reduce toluene pollution in indoor air.
中文翻译:
表达 AtNDPK2 基因的转基因紫菀中外源甲苯气体的增强解毒作用
已知拟南芥核苷二磷酸激酶 2 (AtNDPK2) 基因可调节细胞氧化还原状态,并增强植物对多种压力源的耐受性。在这项研究中,我们在胁迫诱导型启动子 SWPA2 下将 AtNDPK2 转移到 Ardisia pusilla 中,以增强植物对甲苯气体的解毒能力。用 AtNDPK2 和 NPTII 基因特异性引物通过 PCR 分析确认了 30 种转基因 A.pusilla 品系。此外,通过Southern印迹分析进一步证实了四个转基因A.pusilla品系以验证基因拷贝数。三个转基因品系显示单拷贝转基因插入,一个转基因品系具有两个转基因插入。为了测试暴露于和未暴露于甲苯处理的转基因 A. pusilla 品系中 AtNDPK2 的基因表达,进行了 qRT-PCR 分析。AtNDPK2基因在甲苯处理的转基因植物中的表达显着高于未受甲苯处理的转基因植物。最后,我们测量了暴露于甲苯污染空气中的转基因和非转基因 A.pusilla 品系的甲苯去除效率。在所有时间点,转基因和非转基因 A.pusilla 品系之间存在统计学显着差异(p < 0.001)。在接触甲苯 3 小时后,转基因 A. pusilla 品系 NDPK2-12-4 的甲苯去除效率最高(797.33 ± 59.41 µg m−3 cm−2 叶面积),而非转基因品系几乎没有甲苯去除效率(206.2 ± 31.19 µg m−3 cm−2 叶面积)。这些结果表明,解毒甲苯气体的能力与 A. pusilla 中的 AtNDPK2 基因有关。
更新日期:2020-08-26
中文翻译:
表达 AtNDPK2 基因的转基因紫菀中外源甲苯气体的增强解毒作用
已知拟南芥核苷二磷酸激酶 2 (AtNDPK2) 基因可调节细胞氧化还原状态,并增强植物对多种压力源的耐受性。在这项研究中,我们在胁迫诱导型启动子 SWPA2 下将 AtNDPK2 转移到 Ardisia pusilla 中,以增强植物对甲苯气体的解毒能力。用 AtNDPK2 和 NPTII 基因特异性引物通过 PCR 分析确认了 30 种转基因 A.pusilla 品系。此外,通过Southern印迹分析进一步证实了四个转基因A.pusilla品系以验证基因拷贝数。三个转基因品系显示单拷贝转基因插入,一个转基因品系具有两个转基因插入。为了测试暴露于和未暴露于甲苯处理的转基因 A. pusilla 品系中 AtNDPK2 的基因表达,进行了 qRT-PCR 分析。AtNDPK2基因在甲苯处理的转基因植物中的表达显着高于未受甲苯处理的转基因植物。最后,我们测量了暴露于甲苯污染空气中的转基因和非转基因 A.pusilla 品系的甲苯去除效率。在所有时间点,转基因和非转基因 A.pusilla 品系之间存在统计学显着差异(p < 0.001)。在接触甲苯 3 小时后,转基因 A. pusilla 品系 NDPK2-12-4 的甲苯去除效率最高(797.33 ± 59.41 µg m−3 cm−2 叶面积),而非转基因品系几乎没有甲苯去除效率(206.2 ± 31.19 µg m−3 cm−2 叶面积)。这些结果表明,解毒甲苯气体的能力与 A. pusilla 中的 AtNDPK2 基因有关。