Breast cancer is the malignant tumor with the highest incidence in women. Nowadays, the objects in vitro of models of this disease are mainly from breast cancer cell lines and patient-derived patient-derived xenograft (PDX). However, there is a significant gap between traditional cell lines and breast cancer solid tumors, meanwhiles, PDX is not highly consistent with patients due to different species. As a techonlogy, obtaining patient-derived tumor cells, combined with three-dimensional culture technology, adding cytokines that promotes the proliferation of breast cancer stem cells and inhibit their apoptosis, breast cancer organoids form a structure in vitro which is similar to tumor in the body. This model can not only study the occurrence and envolution of breast cancer, but is more prominent in clinical application. screening drugs by high-throughput, personalized treatment, textingtoxicity and immunotherapy. This article will review the breast cancer organoids, in evolution, source, culture system and clinical applications.

中文翻译：

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乳腺癌类器官的研究进展及临床应用。

乳腺癌是女性中发病率最高的恶性肿瘤。如今，该疾病模型的体外对象主要来自乳腺癌细胞系和患者来源的患者来源异种移植物（PDX）。然而，传统的细胞系与乳腺癌实体瘤之间存在着巨大的差距，同时，PDX由于种类不同，与患者的一致性也不高。作为一项技术，获得患者来源的肿瘤细胞，并结合三维培养技术，添加促进乳腺癌干细胞增殖并抑制其凋亡的细胞因子，乳腺癌类器官在体外形成结构这类似于体内的肿瘤。该模型不仅可以研究乳腺癌的发生和发展，而且在临床应用中更为突出。通过高通量，个性化治疗，短信毒性和免疫疗法筛选药物。本文将回顾乳腺癌的类器官，在进化，来源，培养系统和临床应用方面。