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Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer.
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-08-25 , DOI: 10.2147/ijn.s256760
Claudia C Barrera 1 , Helena Groot 1 , Watson L Vargas 2 , Diana M Narváez 1
Affiliation  

Purpose: Expanded research on the biomedical applications of graphene has shown promising results, although interactions between cells and graphene are still unclear. The current study aims to dissect the cellular and molecular effects of graphene nanocomposite in photothermal therapy against cancer, and to evaluate its efficacy.
Methods: In this study, a reduced graphene oxide and iron oxide (rGO-Fe3O4) nanocomposite was obtained by chemical synthesis. The nanocomposite was fully characterized by Raman spectroscopy, TEM, VSM and thermal profiling. Cell-nanocomposite interaction was evaluated by confocal microscopy and viability assays on cancer cell line HeLa. The efficacy of the thermal therapy and changes in gene expression of Bcl-2 and Hsp70 was assessed.
Results: The resulting rGO-Fe3O4 nanocomposite exhibited superparamagnetic properties and the capacity to increase the surrounding temperature by 18– 20°C with respect to the initial temperature. The studies of cell-nanocomposite interaction showed that rGO-Fe3O4 attaches to cell membrane but there is a range of concentration at which the nanomaterial preserves cell viability. Photothermal therapy reduced cell viability to 32.6% and 23.7% with 50 and 100 μg/mL of nanomaterial, respectively. The effect of treatment on the molecular mechanism of cell death demonstrated an overexpression of anti-apoptotic proteins Hsp70 and Bcl-2 as an initial response to the therapy and depending on the aggressiveness of the treatment.
Conclusion: The results of this study contribute to understanding the interactions between cell and graphene and support its application in photothermal therapy against cancer due to its promising results.

Keywords: reduced graphene oxide, iron oxide, photothermal therapy, cell viability, anti-apoptotic genes, molecular effect


中文翻译:

还原氧化石墨烯/Fe3O4 纳米复合材料在光热治疗癌症中的功效和分子效应。

目的:关于石墨烯生物医学应用的扩展研究已显示出可喜的结果,尽管细胞与石墨烯之间的相互作用仍不清楚。目前的研究旨在剖析石墨烯纳米复合材料在光热治疗癌症中的细胞和分子效应,并评估其疗效。
方法:在本研究中,还原氧化石墨烯和氧化铁(rGO-Fe 3 O 4) 纳米复合材料是通过化学合成获得的。纳米复合材料通过拉曼光谱、TEM、VSM 和热曲线进行了充分表征。通过共聚焦显微镜和癌细胞系 HeLa 的活力测定评估细胞-纳米复合材料的相互作用。评估了热疗的功效以及 Bcl-2 和 Hsp70 基因表达的变化。
结果:得到的 rGO-Fe 3 O 4纳米复合材料表现出超顺磁性,并且能够将环境温度相对于初始温度提高 18-20°C。细胞-纳米复合物相互作用的研究表明,rGO-Fe 3 O 4附着在细胞膜上,但纳米材料在一定浓度范围内可以保持细胞活力。光热疗法分别使用 50 和 100 μg/mL 的纳米材料将细胞活力降低至 32.6% 和 23.7%。治疗对细胞死亡分子机制的影响证明了抗凋亡蛋白 Hsp70 和 Bcl-2 的过表达作为对治疗的初始反应,并且取决于治疗的积极性。
结论:这项研究的结果有助于理解细胞和石墨烯之间的相互作用,并支持其在抗癌光热疗法中的应用,因为它的结果令人鼓舞。

关键词:还原氧化石墨烯,氧化铁,光热疗法,细胞活力,抗凋亡基因,分子效应
更新日期:2020-08-25
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