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Fabrication of Photo-Crosslinkable Poly(Trimethylene Carbonate)/Polycaprolactone Nanofibrous Scaffolds for Tendon Regeneration.
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-08-25 , DOI: 10.2147/ijn.s246966 Xing Li 1, 2 , Honglin Chen 3 , Shuting Xie 1, 2 , Ning Wang 3 , Sujuan Wu 1, 2 , Yuyou Duan 3 , Minmin Zhang 4 , Lingling Shui 1, 2, 4
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-08-25 , DOI: 10.2147/ijn.s246966 Xing Li 1, 2 , Honglin Chen 3 , Shuting Xie 1, 2 , Ning Wang 3 , Sujuan Wu 1, 2 , Yuyou Duan 3 , Minmin Zhang 4 , Lingling Shui 1, 2, 4
Affiliation
Background: The treatment of tendon injuries remains a challenging problem in clinical due to their slow and insufficient natural healing process. Scaffold-based tissue engineering provides a promising strategy to facilitate tendon healing and regeneration. However, many tissue engineering scaffolds have failed due to their poor and unstable mechanical properties. To address this, we fabricated nanofibrous polycaprolactone/methacrylated poly(trimethylene carbonate) (PCL/PTMC-MA) composite scaffolds via electrospinning.
Materials and Methods: PTMC-MA was characterized by nuclear magnetic resonance. Fiber morphology of composite scaffolds was evaluated using scanning electron microscopy. The monotonic tensile test was performed for determining the mechanical properties of composite scaffolds. Cell viability and collagen deposition were assessed via PrestoBlue assay and enzyme-linked immunosorbent assay, respectively.
Results: These PCL/PTMC-MA composite scaffolds had an increase in mechanical properties as PTMC-MA content increase. After photo-crosslinking, they showed further enhanced mechanical properties including creep resistance, which was superior to pure PCL scaffolds. It is worth noting that photo-crosslinked PCL/PTMC-MA (1:3) composite scaffolds had a Young’s modulus of 31.13 ± 1.30 MPa and Max stress at break of 23.80 ± 3.44 MPa that were comparable with the mechanical properties of native tendon (Young’s modulus 20– 1200 MPa, max stress at break 5– 100 MPa). In addition, biological experiments demonstrated that PCL/PTMC-MA composite scaffolds were biocompatible for cell adhesion, proliferation, and differentiation.
中文翻译：
制备用于肌腱再生的光交联聚（三亚甲基碳酸酯）/聚己内酯纳米纤维支架。
背景：肌腱损伤的治疗由于其自然愈合过程缓慢且不足，在临床上仍然是一个具有挑战性的问题。基于支架的组织工程为促进肌腱愈合和再生提供了一种有前途的策略。然而，许多组织工程支架由于其较差和不稳定的机械性能而失败。为了解决这个问题，我们通过静电纺丝制造了纳米纤维聚己内酯/甲基丙烯酸化聚（碳酸三亚甲基酯）（PCL/PTMC-MA）复合支架。
材料和方法：PTMC-MA 的特征是核磁共振。使用扫描电子显微镜评估复合支架的纤维形态。进行单调拉伸试验以确定复合支架的力学性能。分别通过 PrestoBlue 测定和酶联免疫吸附测定评估细胞活力和胶原沉积。
结果：这些 PCL/PTMC-MA 复合支架的机械性能随着 PTMC-MA 含量的增加而增加。光交联后，它们显示出进一步增强的机械性能，包括抗蠕变性，优于纯 PCL 支架。值得注意的是，光交联 PCL/PTMC-MA (1:3) 复合支架的杨氏模量为 31.13 ± 1.30 MPa，最大断裂应力为 23.80 ± 3.44 MPa，与天然肌腱的力学性能相当。杨氏模量 20–1200 MPa，最大断裂应力 5–100 MPa)。此外，生物学实验表明，PCL/PTMC-MA复合支架对细胞粘附、增殖和分化具有生物相容性。
更新日期：2020-08-25
Materials and Methods: PTMC-MA was characterized by nuclear magnetic resonance. Fiber morphology of composite scaffolds was evaluated using scanning electron microscopy. The monotonic tensile test was performed for determining the mechanical properties of composite scaffolds. Cell viability and collagen deposition were assessed via PrestoBlue assay and enzyme-linked immunosorbent assay, respectively.
Results: These PCL/PTMC-MA composite scaffolds had an increase in mechanical properties as PTMC-MA content increase. After photo-crosslinking, they showed further enhanced mechanical properties including creep resistance, which was superior to pure PCL scaffolds. It is worth noting that photo-crosslinked PCL/PTMC-MA (1:3) composite scaffolds had a Young’s modulus of 31.13 ± 1.30 MPa and Max stress at break of 23.80 ± 3.44 MPa that were comparable with the mechanical properties of native tendon (Young’s modulus 20– 1200 MPa, max stress at break 5– 100 MPa). In addition, biological experiments demonstrated that PCL/PTMC-MA composite scaffolds were biocompatible for cell adhesion, proliferation, and differentiation.
中文翻译：
制备用于肌腱再生的光交联聚（三亚甲基碳酸酯）/聚己内酯纳米纤维支架。
背景：肌腱损伤的治疗由于其自然愈合过程缓慢且不足，在临床上仍然是一个具有挑战性的问题。基于支架的组织工程为促进肌腱愈合和再生提供了一种有前途的策略。然而，许多组织工程支架由于其较差和不稳定的机械性能而失败。为了解决这个问题，我们通过静电纺丝制造了纳米纤维聚己内酯/甲基丙烯酸化聚（碳酸三亚甲基酯）（PCL/PTMC-MA）复合支架。
材料和方法：PTMC-MA 的特征是核磁共振。使用扫描电子显微镜评估复合支架的纤维形态。进行单调拉伸试验以确定复合支架的力学性能。分别通过 PrestoBlue 测定和酶联免疫吸附测定评估细胞活力和胶原沉积。
结果：这些 PCL/PTMC-MA 复合支架的机械性能随着 PTMC-MA 含量的增加而增加。光交联后，它们显示出进一步增强的机械性能，包括抗蠕变性，优于纯 PCL 支架。值得注意的是，光交联 PCL/PTMC-MA (1:3) 复合支架的杨氏模量为 31.13 ± 1.30 MPa，最大断裂应力为 23.80 ± 3.44 MPa，与天然肌腱的力学性能相当。杨氏模量 20–1200 MPa，最大断裂应力 5–100 MPa)。此外，生物学实验表明，PCL/PTMC-MA复合支架对细胞粘附、增殖和分化具有生物相容性。
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