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m1A Regulator TRMT10C Predicts Poorer Survival and Contributes to Malignant Behavior in Gynecological Cancers.
DNA and Cell Biology ( IF 3.1 ) Pub Date : 2020-10-02 , DOI: 10.1089/dna.2020.5624
Qingying Wang 1 , Qinyi Zhang 2 , Yanjuan Huang 3 , Jiawen Zhang 1
Affiliation  

N1-methyladenosine (m1A) is an important post-transcriptional modification in RNA, and plays critical roles in cellular functions. However, the relationship between m1A regulators and clinical significance of gynecological cancers remains unknown. In this study, we systematically analyzed RNA-seq and clinical data from several public database. Cell proliferation and migration assays were performed to verify the function of the m1A writer TRMT10C in cancer cells. We observed genetic alterations and dysregulated expressions of m1A regulators in gynecological cancer samples. We demonstrated that several m1A regulators could serve as prognostic biomarkers for gynecological cancer patients. The high correlations among the expression of m1A, N6-methyladenosine (m6A), and 5mC regulators were also revealed. Gene set enrichment analysis indicated that the mechanism of TRMT10C in regulating tumorigenesis was related to a variety of cancer-related pathways. Moreover, silencing TRMT10C suppressed the proliferation, colony formation, and migration of ovarian cancer and cervical cancer cells. In summary, our results highlight the importance of m1A regulators in regulating oncogenesis, and indicate that targeting specific m1A regulators might be a potential therapeutic strategy for gynecological cancers.

中文翻译:

m1A 调节剂 TRMT10C 可预测较差的存活率并导致妇科癌症的恶性行为。

N1-甲基腺苷 (m 1 A) 是 RNA 中重要的转录后修饰,在细胞功能中起着关键作用。然而,m 1 A 调节剂与妇科癌症的临床意义之间的关系仍然未知。在这项研究中,我们系统地分析了来自几个公共数据库的 RNA-seq 和临床数据。进行细胞增殖和迁移测定以验证 m 1 A writer TRMT10C 在癌细胞中的功能。我们观察到妇科癌症样本中 m 1 A 调节因子的基因改变和表达失调。我们证明了几个 m 1调节剂可以作为妇科癌症患者的预后生物标志物。还揭示了 m 1 A、N6-甲基腺苷 (m 6 A) 和 5mC 调节因子的表达之间的高度相关性。基因集富集分析表明,TRMT10C调控肿瘤发生的机制与多种癌症相关通路有关。此外,沉默 TRMT10C 抑制了卵巢癌和宫颈癌细胞的增殖、集落形成和迁移。总之,我们的结果强调了 m 1 A 调节剂在调节肿瘤发生中的重要性,并表明靶向特定的 m 1 A 调节剂可能是妇科癌症的潜在治疗策略。
更新日期:2020-10-06
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