In a context of rising demand for sustainable antiaging interventions, fruit processing by-products are a promising source of bioactive compounds for the production of antiaging dietary supplements. Piquiá (Caryocar villosum) is a native Amazonian fruit consisting of 65% nonedible shells. In the present study, the phytochemical profile of a hydroalcoholic extract of piquiá shells (CV) was characterized by LC-MS/MS analysis. Its antioxidant and antiaging activities were investigated using the nematode Caenorhabditis elegans as an in vivo model. CV is mainly composed by hydrolysable tannins and triterpenoid saponins. The extract enhanced stress resistance of wild-type and mutant worms by reducing the intracellular levels of reactive oxygen species (ROS) and by increasing their survival against a lethal dose of the prooxidant juglone. These effects involved the upregulation of sod-3 and downregulation of gst-4 and hsp-16.2, studied through the GFP fluorescent reporter intensity and at the transcriptional level by qRT-PCR analysis. CV extended the lifespan of wild-type worms in a DAF-16/FoxO- and SKN-1/Nrf-dependent manner. Taken together, our findings indicate piquiá shells as potential candidates for nutraceutical applications. Further studies are needed to validate the relevance of our findings to antiaging interventions in humans.

中文翻译：

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PiquiáShells（Caryocar villosum）：秀丽隐杆线虫的一种具有抗氧化和抗衰老特性的水果副产品。

在对可持续抗衰老干预措施的需求不断增长的背景下，水果加工副产品是生产抗衰老膳食补充剂的有希望的生物活性化合物来源。Piquiá（Caryocar villosum）是一种亚马逊原生水果，由65％不可食用的贝壳组成。在本研究中，通过LC-MS / MS分析表征了皮壳水醇提取物（CV）的植物化学特征。使用线虫秀丽隐杆线虫（Caenorhabditis elegans）作为体内研究了其抗氧化和抗衰老活性。模型。CV主要由可水解的单宁和三萜皂苷组成。该提取物通过降低细胞内活性氧（ROS）的水平，并通过增加它们对致死剂量的促氧化剂朱古龙的存活率来增强野生型和突变型蠕虫的抗逆性。这些影响涉及sod-3的上调和gst-4和hsp-16.2的下调通过qRT-PCR分析，通过GFP荧光报告基因强度和转录水平进行研究。CV以DAF-16 / FoxO-和SKN-1 / Nrf依赖的方式延长了野生蠕虫的寿命。综上所述，我们的研究结果表明，piquiá贝壳可作为保健食品的潜在候选产品。需要进一步的研究来验证我们的发现与人类抗衰老干预措施的相关性。