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Wound Healing Activity of Crassocephalum crepidioides (Benth.) S. Moore. Leaf Hydroethanolic Extract.
Oxidative Medicine and Cellular Longevity ( IF 7.310 ) Pub Date : 2020-08-25 , DOI: 10.1155/2020/2483187
Nguyen Minh Can 1, 2, 3 , Dang Thi Phuong Thao 1, 2
Affiliation  

Crassocephalum crepidioides (Benth.) S. Moore. has been used to treat small wounds by minority people in Lam Dong, Vietnam. However, there has been no scientific evidences about its wound healing activity. This study is aimed at evaluating the wound healing activity of Crassocephalum crepidioides hydroethanolic extract via its antioxidant and anti-inflammation activities and healing capability on a mouse excision wound model. Crassocephalum crepidioides hydroethanolic extract (CCLE) at a dose of 50 mg/kg/day reduced the wound closure time about 3.5 days, compared to vehicle treatment. The granulation tissue on day 7 after surgery from the treated group showed a 2.8-fold decrease in the density of inflammatory cells, 1.9-fold increase in the fibroblast density, and a higher number of blood vessels. Real-time PCR analysis indicated that the mRNA expression level of NF-κB1 and TNF-α mRNA in CCLE-treated wounds decreased by 4.6 and 3.3 times, respectively, while TGF-β1 and VEGF were found to increase by 3.3 and 2.4 times, respectively. Our experimental data provided proofs of Crassocephalum crepidioides leaf wound healing activity due to its antioxidant, anti-inflammation, fibroblast proliferation, wound contraction, and angiogenesis effects.

中文翻译:

Crassocephalum crepidioides(Benth。)S. Moore的伤口愈合活性。叶氢乙醇提取物。

Crassocephalum crepidioides(Benth。)S. Moore。越南林同省的少数民族已经使用这种方法来治疗小伤口。但是,没有关于其伤口愈合活性的科学证据。这项研究旨在通过抗氧化和抗炎活性以及在小鼠切除伤口模型上的愈合能力来评估Crassocephalum crepidioides氢乙醇提取物的伤口愈合活性。Crassocephalum crepidioides与媒介物治疗相比,剂量为50 mg / kg / day的氢乙醇提取物(CCLE)减少了约3.5天的伤口闭合时间。手术后第7天,治疗组的肉芽组织炎症细胞密度降低了2.8倍,成纤维细胞密度增加了1.9倍,血管数量增加。实时PCR分析表明,NF-κB的mRNA表达水平κ B1和TNF- α在CCLE治疗伤口的mRNA减少了4.6和3.3倍,分别,而TGF- β 1和VEGF被发现了3.3和2.4,以增加次。我们的实验数据提供了Crassocephalum crepidioides的证据。 由于其抗氧化剂,抗炎作用,成纤维细胞增殖,伤口收缩和血管生成作用,因此具有伤口愈合作用。
更新日期:2020-08-25
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