Driven by complex and interconnected factors, including population growth, climate change, and geopolitics, infectious diseases represent one of the greatest healthcare challenges of the 21st century. Diagnostic technologies are the first line of defense in the fight against infectious disease, providing critical information to inform epidemiological models, track diseases, decide treatment choices, and ultimately prevent epidemics. The diagnosis of infectious disease at the genomic level using nucleic acid disease biomarkers has proven to be the most effective approach to date. Such methods rely heavily on enzymes to specifically amplify or detect nucleic acids in complex samples, and significant effort has been exerted to harness the power of enzymes for in vitro nucleic acid diagnostics. Unfortunately, significant challenges limit the potential of enzyme-assisted nucleic acid diagnostics, particularly when translating diagnostic technologies from the lab toward the point-of-use or point-of-care. Herein, we discuss the current state of the field and highlight cross-disciplinary efforts to solve the challenges associated with the successful deployment of this important class of diagnostics at or near the point-of-care.

中文翻译：

---

用于传染病诊断的酶辅助核酸检测：走向护理点。

在人口增长、气候变化和地缘政治等复杂且相互关联的因素的推动下，传染病是 21 世纪最大的医疗保健挑战之一。诊断技术是对抗传染病的第一道防线，为流行病学模型提供关键信息、跟踪疾病、决定治疗选择并最终预防流行病。使用核酸疾病生物标志物在基因组水平上诊断传染病已被证明是迄今为止最有效的方法。此类方法严重依赖酶来特异性扩增或检测复杂样品中的核酸，并且已经付出了巨大的努力来利用酶的力量进行体外核酸诊断。不幸的是，重大挑战限制了酶辅助核酸诊断的潜力，特别是在将诊断技术从实验室转化为使用点或护理点时。在此，我们讨论该领域的现状，并强调跨学科的努力，以解决与在护理点或护理点附近成功部署此类重要诊断相关的挑战。