Benzodiazepines are a commonly prescribed class of drugs that have the potential for abuse. The Palm Beach County Sheriff’s Office received drug seizure submissions that included novel and/or nonroutine benzodiazepines of increasing prevalence from 2017 to 2019. This prompted the development of a method of analysis for these compounds in biological specimens. The method tests for 16 novel and nonroutine benzodiazepines and suvorexant in whole blood by liquid chromatography–tandem mass spectrometry (LC–MS-MS). The target analytes included bromazepam, clobazam, clonazolam, clotiazepam, diclazepam, estazolam, etizolam, flualprazolam, flubromazepam, flubromazolam, loprazolam, lormetazepam, phenazepam, prazepam, suvorexant, tetrazepam and triazolam. The method uses 200 µL of sample, protein precipitation and an instrument run-time of 8 min. The limit of detection was either 1 or 5 ng/mL and the limit of quantitation was either 5 or 25 ng/mL depending on the analyte. The method was validated for quantitative analysis for 15 out of the 17 analytes. Flubromazepam and prazepam were validated for qualitative identification only. A quadratic calibration model (r² > 0.990) with 1/x weighting was used for all analytes for quantitative analysis. The calibration range was either 5–100 or 25–500 ng/mL depending on the analyte. The coefficient of variation of replicate analyses was within 14% and bias was within ±14%. The method provides a sensitive, efficient and robust procedure for the quantitation and/or qualitative identification of select novel and nonroutine benzodiazepines and suvorexant using LC–MS-MS and a sample volume of 200 µL.

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LC-MS-MS检测新型和非常规苯二氮卓类药物和苏泊生

苯二氮卓类药物是一种常见处方药，可能会被滥用。棕榈滩县警长办公室收到了缉毒报告，其中包括从2017年到2019年患病率上升的新型和/或非常规苯二氮卓类药物。这促使人们开发了一种分析生物样本中这些化合物的方法。该方法通过液相色谱-串联质谱法（LC-MS-MS）对全血中的16种新型和非常规苯二氮卓类和苏威生酯进行了测试。目标分析物包括溴马西m，克罗巴am，可乐唑仑，克洛替西am，地克西p，依他唑仑，依替唑仑，氟普拉唑仑，氟溴马西m，氟溴马唑仑，洛哌唑仑，洛美他西,、苯并西,、吡raz西m，苏弗罗西坦，四西and和。该方法使用200 µL样品，蛋白质沉淀和仪器运行时间为8分钟。根据分析物的不同，检测限为1或5 ng / mL，定量限为5或25 ng / mL。该方法经验证可用于17种分析物中的15种的定量分析。氟西西epa和吡raz西m仅用于定性鉴定。二次校准模型（r  / x权重为r ² > 0.990）用于所有分析物进行定量分析。根据分析物的不同，校准范围为5–100或25–500 ng / mL。重复分析的变异系数在14％以内，偏差在±14％以内。该方法使用LC-MS-MS和200 µL的样品量，为定量筛选和/或定性鉴定新颖的和非常规的苯二氮杂和suvorexant提供了一种灵敏，高效且可靠的方法。