The ‘first 1,000 days of life’ determine the gut microbiota composition and can have long-term health consequences. In this study, the simulator of the human intestinal microbial ecosystem (SHIME^®) model, which represents the main functional sections of the digestive tract, was chosen to study the microbiota of young children. The aim of this study was to reproduce the digestive process of toddlers and their specific colonic environment. The ascending, transverse and descending colons of SHIME^® model were inoculated with feces from 3 donors aged between 1 and 2 years old, in 3 separate runs. For each run, samples from colon vessels were collected at days 14, 21 and 28 after microbiota stabilization period. Short chain fatty acid concentrations determined by HPLC showed that microbiota obtained in SHIME^® model shared characteristics between adults and infants. In addition, microbial diversity and bacterial populations determined by 16S rDNA amplicon sequencing were specific to each colon vessel. In conclusion, the SHIME^® model developed in this study seemed well adapted to evaluate prebiotic and probiotic impact on the specific microbiota of toddlers, or medicine and endocrine disruptor metabolism. Moreover, this study is the first to highlight some biofilm development in in vitro gastrointestinal modelling systems.

中文翻译：

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SHIME®幼儿模型用于研究幼儿的微生物群。

“生命的前1000天”决定了肠道菌群的组成，并可能对健康产生长期影响。在这项研究中，人体肠道微生物生态系统（SHIME的模拟器^®）模型，它代表了消化道的主要功能部分，被选为研究幼儿的微生物。这项研究的目的是再现幼儿的消化过程及其特定的结肠环境。在升结肠，横结肠和SHIME的降结肠^®在3个独立的实验中，分别从3个年龄在1至2岁之间的供体的粪便中接种模型。对于每次运行，在微生物群稳定期后的第14、21和28天从结肠血管收集样品。短链脂肪酸浓度测定通过HPLC显示在SHIME获得的微生物群^®成人和婴儿之间模型共享特征。此外，通过16S rDNA扩增子测序确定的微生物多样性和细菌种群对每个结肠血管具有特异性。总之，SHIME ^®在这项研究中开发的模式似乎很好地适应，以评估对幼儿，或药物和内分泌干扰物的代谢的特定菌群益生元和益生菌的影响。而且，这项研究是第一个突出生物膜发展的研究。体外胃肠道建模系统。