A biotin-HSA-DDA-TCPP molecule, which can be used as a photodynamic therapeutic agent and drug carrier, was synthesized. The molecule can self-assemble into spherical aggregates, which can be loaded with Dox to form biotin-HSA-DDA-TCPP-Dox nanoparticles in aqueous solution, and the Dox loading efficiency was 86.6 ± 1.76%. The Dox’s release behavior was pH responsive and has a sustained release. Cell experiments showed that biotin-HSA-DDA-TCPP-Dox nanoparticles could effectively induce cancer cell apoptosis to exert anticancer and photodynamic therapy effects. The results of animal experiments, tissue sections, and blood biochemistry tests showed that the biotin-HSA-DDA-TCPP-Dox nanoparticles could exert the effect of photodynamic therapy and antitumor, which is similar to Dox after laser irradiation, and achieve a synergistic antitumor effect. The nanoparticles can significantly reduce the Dox toxicity and increase the circulation time of the drug in the body. In summary, the biotin-HSA-DDA-TCPP molecule, which combines the advantages of photodynamic therapy and drug carrier, has great potential in clinical application.

中文翻译：

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基于生物素-HSA-DDA-TCPP分子的药物递送系统和光动力治疗试剂的构建及协同抗肿瘤作用的应用

合成了一种可作为光动力治疗剂和药物载体的生物素-HSA-DDA-TCPP分子。该分子可自组装成球形聚集体，可在水溶液中负载Dox形成生物素-HSA-DDA-TCPP-Dox纳米粒子，Dox负载效率为86.6±1.76%。Dox 的释放行为是对 pH 值敏感的并且具有持续释放。细胞实验表明，生物素-HSA-DDA-TCPP-Dox纳米粒子可有效诱导癌细胞凋亡，发挥抗癌和光动力治疗作用。动物实验、组织切片、血液生化试验结果表明，生物素-HSA-DDA-TCPP-Dox纳米粒在激光照射后可发挥与Dox相似的光动力治疗和抗肿瘤作用，达到协同抗肿瘤的效果。影响。纳米颗粒可以显着降低Dox毒性，增加药物在体内的循环时间。综上所述，结合了光动力疗法和药物载体优势的生物素-HSA-DDA-TCPP分子在临床应用中具有巨大的潜力。