Abstract

The employment of an 8-hydroxyquinoline carbohydrazone tetradentate ligand, 8-hydroxyquinoline-2-carboxaldehyde-(benzoyl)hydrazone (H₂L), HoCl₃·6H₂O and Et₃N has led to the formation of dinuclear Ho(III) complex [Ho₂(HL)₂((PhO)₂PO₂)₄]·2(CH₃OH) (1) in the presence of excess amount of bridging diphenyl phosphate anion, (PhO)₂PO²⁻. The biological studies of the title compound were conducted to evaluate the treatment activities on mania. The ELISA detection kit was used to detect the content of the neuroregulin1 (NRG1) in the hippocampus. The RT-PCR was performed to evaluate the relative expression levels of the ErbB4 on the noradrenergic neurons. Furthermore, the mechanism of the complex interacting with the protein is identified by the molecular docking simulation.

摘要

使用8-羟基喹啉碳tetra四齿配体，8-羟基喹啉-2-羧醛-（苯甲酰基）hydr（H ₂ L），HoCl ₃ ·6H ₂ O和Et ₃ N导致了双核Ho（III）的形成。在过量桥联二苯基磷酸根阴离子（PhO）₂ PO ²⁻的存在下形成[Ho ₂（HL）₂（（PhO）₂ PO ₂）₄ ]·2（CH ₃ OH）（1）。进行了标题化合物的生物学研究，以评估对躁狂症的治疗活性。ELISA检测试剂盒用于检测海马中神经调节蛋白1（NRG1）的含量。进行RT-PCR以评估去甲肾上腺素能神经元上ErbB4的相对表达水平。此外，通过分子对接模拟确定了复合物与蛋白质相互作用的机理。