Abstract

Zinc oxide nanoparticles (ZnONPs) have been widely applied as an efficient anticancer instrument. In the current study, we aimed to green-synthesize ZnONPs to investigate its antitumor impacts on the human breast (MCF7 and MDA-MB231) and colon (HT-29) cancer cell lines. They were synthesized using the Ferula assa-foetida and characterized by the XRD, FTIR, TEM, and SEM techniques subsequently. Then, their cytotoxic impacts were studied on cancer (MCF7, MDA-MB231, and HT-29) and normal (Huvec) cell lines. Also, the apoptotic and antioxidant activities of the nanoparticles were detected and verified. The ∼40-nm ZnONPs significantly induce the cell-selective cytotoxic and apoptotic (BAX overexpression, Sub G1 peaks enhancement, and fluorescent stained apoptotic cells) impacts. The findings suggest the novel biocompatible ZnONPs can be useful as a safe natural apoptosis inducer in human breast and colon cancer cells. However, further genes’ expression and cell lines are required to verify its cell-selective antitumor activity.

抽象的

氧化锌纳米颗粒（ZnONPs）已被广泛用作一种有效的抗癌仪器。在当前的研究中，我们旨在绿色合成ZnONP，以研究其对人乳腺癌（MCF7和MDA-MB231）和结肠癌（HT-29）癌细胞系的抗肿瘤作用。它们是用费拉草（Ferula assa-foetida）合成的，并随后通过XRD，FTIR，TEM和SEM技术进行了表征。然后，研究了它们对癌细胞（MCF7，MDA-MB231和HT-29）和正常（Huvec）细胞系的细胞毒性影响。而且，检测并验证了纳米颗粒的凋亡和抗氧化活性。约40 nm的ZnONPs明显诱导细胞选择性细胞毒性和凋亡（BAX过表达，Sub G1峰增强和荧光染色的凋亡细胞）的影响。这些发现表明，新型的生物相容性ZnONPs可以用作人类乳腺癌和结肠癌细胞中安全的自然凋亡诱导剂。但是，需要进一步的基因表达和细胞系来验证其细胞选择性抗肿瘤活性。