Abstract

To investigate the effects of escin (ES) on acute damage induced by alkylating agent, experimental rats were injected with cyclophosphamide (CPM) to cause liver damage. The animals were divided into four groups: Control Group, CPM (200 mg/kg), ES (10 mg/kg), CPM, and ES Groups. Immunohistopathological, hepatic histopathological, and biochemical changes were analyzed. The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), malondyaldehyde (MDA), glutathion (GSH), total oxidant status (TOS) and total antioxidant status (TAS) in serum were all determined. Serum and immunohistopathology analysis revealed that MDA, ALT, AST, LDH, TOC and OSI, caspase-3 and Bax levels had increased while GSH, TAC, Bcl- 2 and OSI levels decreased in CPM Group when compared to Control Group. These findings appear to account for the severe damage detected. In the CPM + ES treated group, positive improvements were found in biochemical parameters as well as in cell-death and tissue-related damage parameters.The results show that ES considerably protects the rat liver against CPM-induced hepatotoxicity thanks to because of its anti-oxidant and anti-apoptotic properties.

摘要

为了研究七叶皂苷（ES）对烷化剂引起的急性损伤的影响，给实验大鼠注射环磷酰胺（CPM）以引起肝损伤。将动物分为四组：对照组、CPM (200 mg/kg)、ES (10 mg/kg)、CPM和ES组。分析免疫组织病理学、肝组织病理学和生化变化。测定血清中天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、乳酸脱氢酶（LDH）、丙二醛（MDA）、谷胱甘肽（GSH）、总氧化状态（TOS）和总抗氧化状态（TAS）的活性。血清和免疫组织病理学分析显示，与对照组相比，CPM 组的 MDA、ALT、AST、LDH、TOC 和 OSI、caspase-3 和 Bax 水平升高，而 GSH、TAC、Bcl-2 和 OSI 水平降低。这些发现似乎解释了检测到的严重损害。在 CPM + ES 治疗组中，生化参数以及细胞死亡和组织相关损伤参数都有积极的改善。结果表明，ES 显着保护大鼠肝脏免受 CPM 诱导的肝毒性，因为它具有抗- 氧化和抗凋亡特性。