当前位置: X-MOL 学术Biotech. Histochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Crocin ameliorates oxidative stress and suppresses renal damage in streptozotocin induced diabetic male rats.
Biotechnic & Histochemistry ( IF 1.6 ) Pub Date : 2020-08-24 , DOI: 10.1080/10520295.2020.1808702
Ayşegül Kapucu 1
Affiliation  

ABSTRACT

Diabetes mellitus (DM) is a prevalent metabolic disorder with complications including nephropathy, neuropathy, retinopathy and peripheral vascular disease. Crocin is a water soluble carotenoid that exhibits strong antioxidant activity. I investigated the potential hypoglycemic and reno-protective effects of crocin for type 1 diabetic male rats using periodic acid-Schiff and hematoxylin staining; metalloproteinase-7 (MMP-7), phosphatase and tensin homolog (PTEN) and phospho-Akt (p-Akt) immunohistochemistry; measurement of blood glucose, malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant and total oxidant status; and oxidative stress index. I used four groups of rats: control (saline treated), diabetic (single dose of 65 mg/kg streptozotocin), crocin (treated with 50 mg/kg) and diabetic + crocin. Crocin decreased levels of MDA, SOD, oxidative stress index and glucose significantly in diabetic animals. Renal damage from DM also was decreased by crocin treatment. MMP-7 and p-Akt immunoreactivity were stronger in the diabetic group compared to other groups, but PTEN immunoreactivity was weaker. Crocin treatment returned MMP-7 and PTEN expression to near normal. The ameliorative effect of crocin is attributed to its stimulation of the antioxidant defense system and its ability to regulate the MMP-7/PTEN/Akt signaling cascade.



中文翻译:

藏红花素改善氧化应激并抑制链脲佐菌素诱导的糖尿病雄性大鼠的肾损伤。

摘要

糖尿病 (DM) 是一种常见的代谢性疾病,其并发症包括肾病、神经病、视网膜病和外周血管疾病。藏红花素是一种水溶性类胡萝卜素,具有很强的抗氧化活性。我使用高碘酸-希夫和苏木精染色研究了藏红花素对 1 型糖尿病雄性大鼠的潜在降血糖和肾脏保护作用;金属蛋白酶-7 (MMP-7)、磷酸酶和张力蛋白同源物 (PTEN) 和磷酸化 Akt (p-Akt) 免疫组织化学;测量血糖、丙二醛 (MDA)、超氧化物歧化酶 (SOD)、总抗氧化剂和总氧化状态;和氧化应激指数。我使用了四组大鼠:对照(盐水治疗)、糖尿病(单剂量 65 毫克/千克链脲佐菌素)、藏红花素(用 50 毫克/千克治疗)和糖尿病 + 藏红花素。藏红花素降低 MDA、SOD、氧化应激指数和葡萄糖在糖尿病动物中显着。藏红花素治疗也减少了 DM 引起的肾损伤。与其他组相比,糖尿病组的 MMP-7 和 p-Akt 免疫反应性更强,但 PTEN 免疫反应性较弱。藏红花素处理使 MMP-7 和 PTEN 的表达接近正常。藏红花素的改善作用归因于其对抗氧化防御系统的刺激及其调节 MMP-7/PTEN/Akt 信号级联的能力。

更新日期:2020-08-24
down
wechat
bug