Modular construction of an autonomous and programmable multi‐functional heterogeneous biochemical circuit that can identify, transform, translate, and amplify biological signals into physicochemical signals based on logic design principles can be a powerful means for the development of a variety of biotechnologies. To explore the conceptual validity, we design a CRISPR‐array‐mediated primer‐exchange‐reaction‐based biochemical circuit cascade, which probes a specific biomolecular input, transform the input into a structurally accessible form for circuit wiring, translate the input information into an arbitrary sequence, and finally amplify the prescribed sequence through autonomous formation of a signaling concatemer. This upstream biochemical circuit is further wired with a downstream electrochemical interface, delivering an integrated bioanalytical platform. We program this platform to directly analyze the genome of SARS‐CoV‐2 in human cell lysate, demonstrating the capability and the utility of this unique integrated system.

中文翻译：

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用于基于高分辨率电化学​​的遗传分析的集成多功能异质生化电路。

模块化构建自主可编程的多功能异质生化电路，可以基于逻辑设计原理识别、转换、翻译和放大生物信号为物理化学信号，可以成为开发各种生物技术的有力手段。为了探索概念的有效性，我们设计了一种基于 CRISPR 阵列介导的引物交换反应的生化电路级联，它探测特定的生物分子输入，将输入转换为电路布线的结构可访问形式，将输入信息转化为任意序列，最后通过自主形成信号串联体放大指定序列。该上游生化电路进一步与下游电化学接口连接，提供集成的生物分析平台。我们对该平台进行编程，以直接分析人类细胞裂解物中的 SARS-CoV-2 基因组，展示了这种独特集成系统的功能和实用性。