This work aimed to prepare a hydrogel film with antibacterial activity and excellent stability in enzymes as potential wound dressing. Using hyaluronic acid, carboxylated chitosan and gentamicin, antibacterial film HA-CC-GS was prepared through intermolecular covalent bonding. Surface morphology of film was evaluated by scanning electron microscope images. The film exhibited good water absorption capacity and appropriate water vapor permeation rate (WVPR), outstanding mechanical properties and remarkable resistance to enzymatic hydrolysis. The biocompatibility of the film was tested by CCK-8 assay, and the results showed that the viability of NIH3T3 cell was not affected by coculture with hydrogel film. In vitro antibacterial test indicated that the synthesized hydrogel film had obvious antibacterial properties. In addition, in vivo studies showed that antibacterial hydrogel film could protect wound from infection and promote wound healing. These results indicated that the prepared antimicrobial hydrogel films by cross-linking had great potential application in skin/tissue repair, suggesting as a hopeful wound dressing.

这项工作旨在制备具有抗菌活性和出色的酶稳定性的水凝胶薄膜，作为潜在的伤口敷料。使用透明质酸，羧化壳聚糖和庆大霉素，通过分子间共价键合制备抗菌膜HA-CC-GS。通过扫描电子显微镜图像评价膜的表面形态。该膜表现出良好的吸水能力和适当的水蒸气渗透率（WVPR），出色的机械性能以及出色的抗酶水解性。用CCK-8法检测了膜的生物相容性，结果表明与水凝胶膜共培养不会影响NIH3T3细胞的活力。体外抗菌试验表明，合成的水凝胶膜具有明显的抗菌性能。此外，体内研究表明，抗菌水凝胶膜可保护伤口免受感染并促进伤口愈合。这些结果表明，通过交联制备的抗菌水凝胶膜在皮肤/组织修复中具有巨大的潜在应用，表明其是希望的伤口敷料。