A new type of immunomagnetic bead based on the metal–organic framework materials (MOFs) and the magnetic core (Fe₃O₄) was prepared for analysis of the mycotoxins in food samples. The MOF conjugated with the monoclonal antibodies (McAbs) was coated on the surface of Fe₃O₄ beads by reversed-phase microemulsion method, which could purify deoxynivalenol (DON), zearalenone (ZEN), T-2, and HT-2 mycotoxins at the same time. The composite (Fe₃O₄@AMP&ZnCl₂@McAbs) was characterized by Transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and Energy dispersive spectroscopy (EDS) mapping. The results showed that the synthesis of the composite was successful. The maximum toxin adsorption capacity per 100 mg of composite was DON 688.26 ng, ZEN 864.98 ng, and T-2/HT-2 2801.80 ng, and in adding recovery experiment, the recovery of four mycotoxins decreased slightly with the increase of usage times, but still maintained a high adsorption rate and stability. For effectiveness comparison and evaluation, the composite and commercial DZTMS-PREP immune affinity column were used to treat three samples of corn, wheat, and oat flour, and the purification effect of the two pretreatment methods on the four toxins was similar.

基于金属-有机骨架材料（MOFs）和磁芯（Fe ₃ O ₄）制备了一种新型的免疫磁珠，用于分析食品中的霉菌毒素。通过反相微乳液法将与单克隆抗体（McAbs）偶联的MOF包被在Fe ₃ O ₄珠的表面，可以纯化脱氧雪茄烯醇（DON），玉米赤霉烯酮（ZEN），T-2和HT-2霉菌毒素同时。复合材料（Fe ₃ O ₄ @ AMP＆ZnCl ₂通过透射电子显微镜（TEM），傅立叶变换红外光谱（FTIR）和能量色散光谱（EDS）作图来表征。结果表明该复合材料的合成是成功的。每100 mg复合物的最大毒素吸附量为DON 688.26 ng，ZEN 864.98 ng和T-2 / HT-2 2801.80 ng，在添加回收率实验中，四种霉菌毒素的回收率随使用时间的增加而略有下降，但仍保持较高的吸附速率和稳定性。为了进行有效性比较和评估，使用复合和商业DZTMS-PREP免疫亲和柱处理了三个玉米，小麦和燕麦粉样品，两种预处理方法对四种毒素的纯化效果相似。