A novel sulfonate-bonded covalent organic polymer (COP_TPBA-BPDA@SA) with mixed-mode interactions of hydrophobic and cation-exchange was synthesized and exploited as sorbent for on-line solid-phase extraction (on-line SPE) of ß₂-receptor agonists. The successful synthesis of COP_TPBA-BPDA@SA was confirmed by the characterization of SEM, XPS and FT-IR. Due to the excellent mixed-mode extraction ability towards the positively charged ß₂-receptor agonists and the good anti-interference performance, COP_TPBA-BPDA@SA was introduced into on-line SPE-HPLC system for selective extraction of ß₂-receptor agonists in pork and pork liver. Via the optimization of the extraction condition, including formic acid percentage and ACN percentage in the sampling solution, the mixed-mode extraction mechanism of COP_TPBA-BPDA@SA was investigated. The elution condition, such as the pH value, formic acid percentage and ACN percentage of the eluent was also optimized for the desired SPE performance. Under the optimized condition, COP_TPBA-BPDA@SA revealed better purification performance than COP_TPBA-BPDA without sulfonating. The LODs for ß₂-receptor agonists were in the range of 0.08–0.22 μg/kg, and the recoveries in different samples at three spiked levels (0.4, 4.0, 8.0 μg/kg) were ranged from 83.2% to 98.5% with RSDs less than 5.2%, which indicated the satisfactory mixed-mode extraction ability of COP_TPBA-BPDA@SA as well as the good applicability of the developed method.

一种新颖的磺酸酯键合的共价有机聚合物（COP _TPBA-BPDA @SA）用疏水性和阳离子交换的混合模式的交互合成和开发为吸附剂对的SS上线固相萃取（在线SPE）₂ -受体激动剂。SEM，XPS和FT-IR的表征证实了COP _TPBA-BPDA @SA的成功合成。由于朝ß带正电的优良混合模式萃取能力₂ -受体激动剂和良好的抗干扰性能，COP _TPBA-BPDA @SA导入上在线SPE-HPLC系统的SS选择性提取₂-猪肉和猪肝中的受体激动剂。通过优化萃取条件，包括采样溶液中的甲酸百分比和乙腈百分比，研究了COP _TPBA-BPDA @SA的混合模式萃取机理。还针对所需的SPE性能优化了洗脱条件，例如洗脱液的pH值，甲酸百分比和ACN百分比。在优化条件下，COP _TPBA-BPDA @SA的纯化性能优于未磺化的COP _TPBA-BPDA。SS的检出限₂受体激动剂的浓度范围为0.08-0.22μg/ kg，三种加标水平（0.4、4.0、8.0μg/ kg）在不同样品中的回收率在83.2％至98.5％之间，RSD小于5.2％，这表明COP _TPBA-BPDA @SA具有令人满意的混合模式提取能力，并且该方法具有良好的适用性。