In the present work we have focused on the Histone Deacetylase (HDAC) control of myeloid cells behavior during Xenopus tail regeneration. Here we show that myeloid differentiation is crucial to modulate the regenerative ability of Xenopus tadpoles in a HDAC activity-dependent fashion. HDAC activity inhibition during the first wave of myeloid differentiation disrupted myeloid cells dynamics in the regenerative bud as well the mRNA expression pattern of myeloid markers, such as LURP, MPOX, Spib and mmp7. We also functionally bridge the spatial and temporal dynamics of lipid droplets, the main platform of lipid mediators synthesis in myeloid cells during the inflammatory response, and the regenerative ability of Xenopus tadpoles. In addition, we showed that 15-LOX activity is necessary during tail regeneration. Taken together our results support a role for the epigenetic control of myeloid behavior during tissue and organ regeneration, which may positively impact translational approaches for regenerative medicine.

在目前的工作中，我们专注于组蛋白脱乙酰酶 (HDAC) 在非洲爪蟾尾部再生过程中对骨髓细胞行为的控制。在这里，我们表明骨髓分化对于以HDAC 活动依赖性方式调节爪蟾蝌蚪的再生能力至关重要。第一波骨髓分化期间的 HDAC 活性抑制破坏了再生芽中的骨髓细胞动力学以及骨髓标志物的 mRNA 表达模式，例如 LURP、MPOX、Spib 和 mmp7。我们还在功能上桥接了脂滴的时空动态、炎症反应过程中骨髓细胞中脂质介质合成的主要平台，以及非洲爪蟾的再生能力蝌蚪。此外，我们发现尾再生过程中需要 15-LOX 活性。综上所述，我们的结果支持在组织和器官再生过程中对骨髓行为进行表观遗传控制的作用，这可能对再生医学的转化方法产生积极影响。