Eucalyptus grandis and Eucalyptus globulus are important species for the Brazilian forestry industry. E. grandis plantations are mainly found in tropical regions, yet E. globulus plants are usually cultivated under moderate to low temperature conditions. As temperature seems to be a key factor for the planting of these species, we revisited our previously generated shotgun proteomics dataset to identify the main patterns of proteome regulation induced by thermal stimulus and to pinpoint specific proteins involved in the environmental response. Large-scale analysis has pointed out the different proteomic responses of E. grandis and E. globulus under temperature stimulus, with 296 proteins considered to be differentially regulated in the stems of Eucalyptus spp. grown at different temperatures. A stringent filtering approach was used to identify the most differentially regulated proteins. Through the stringent criteria, 66 proteins were found to be enriched in the plant species. Cultivation of E. globulus plants in low-temperature conditions induced the highest number of differentially regulated proteins. Additionally, metabolic proteins were mostly down-regulated, while stress-related proteins were majorly up-regulated in both species. Finally, the subset of the most differentially regulated proteins comprised new candidates of protein markers of temperature stress.

桉树和桉树是巴西林业的重要树种。大E. grandis人工林主要分布在热带地区，而E. globulus植物通常在中等至低温条件下种植。由于温度似乎是种植这些物种的关键因素，因此我们重新研究了先前生成的shot弹枪蛋白质组学数据集，以识别热刺激诱导的蛋白质组调控的主要模式，并查明参与环境响应的特定蛋白质。大规模分析指出了E. grandis和E. globulus的不同蛋白质组学反应在温度刺激下，有296种蛋白质被认为在桉树的茎中有不同的调控。在不同的温度下生长。使用严格的过滤方法来鉴定调节最差的蛋白质。通过严格的标准，发现植物物种中富含66种蛋白质。在低温条件下培养的球形肠球菌诱导了最多数量的差异调节蛋白。此外，在两个物种中，代谢蛋白大多被下调，而与压力相关的蛋白则主要上调。最后，调节最差的蛋白质的子集包括温度胁迫的蛋白质标志物的新候选物。