Abstract

Soil salinity is a major abiotic stress that adversely affects crop growth, development and productivity worldwide. In this study, the individual and synergistic roles of putrescine (Put) and spermidine (Spd) in salinity stress tolerance of foxtail millet (Setaria italica L.) was assessed. In the present study, plants treated with combined biogenic amines Put + Spd possess very efficient antioxidant enzyme systems which help to control the uninhibited oxidation and protect the plants from oxidative damage by ROS scavenging. Additionally, lower concentration of Put + Spd under NaCl stress showed reduced hydrogen peroxide, electrolyte leakage and caspase-like activity than control. FTIR analysis underlying the ability of PAs induced tolerance and the chemical bonds of Put + Spd treated plants were reminiscent of control plants. Moreover, histochemical analysis with 2′,7′–dichlorofluorescein diacetate (DCF-DA), 3,3′–Diaminobenzidine (DAB) and nitrotetrazolium blue chloride (NBT) revealed that ROS accumulation was inhibited by combined PAs under salt stress condition. These results showed that Put + Spd significantly improve the endogenous PAs, which enhance high-salinity stress tolerance by detoxifying ROS. For the first time, the synergistic ROS scavenging ability of Put along with Spd was investigated upon salinity tolerance in C4 model foxtail millet crop. Overall, our findings illustrated the implication for improving salinity tolerance of agronomically important crop species.

Graphic abstract

中文翻译：

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多胺对重要 C4 模式作物谷子 (Setaria italica L.) 盐胁迫耐受性的保护作用。

摘要

土壤盐分是一种主要的非生物胁迫，对全世界的作物生长、发育和生产力产生不利影响。本研究探讨腐胺 (Put) 和亚精胺 (Spd) 在谷子 ( Setaria italica ) 耐盐胁迫中的个体和协同作用L.) 进行了评估。在本研究中，用组合生物胺 Put + Spd 处理的植物具有非常有效的抗氧化酶系统，有助于控制不受抑制的氧化并保护植物免受 ROS 清除造成的氧化损伤。此外，与对照相比，NaCl 胁迫下较低浓度的 Put + Spd 显示过氧化氢、电解质泄漏和半胱天冬酶样活性降低。PAs 诱导耐受能力的 FTIR 分析和 Put + Spd 处理植物的化学键让人想起对照植物。此外，用 2',7'-二氯荧光素二乙酸酯 (DCF-DA)、3,3'-二氨基联苯胺 (DAB) 和氯化硝基四唑蓝 (NBT) 进行的组织化学分析表明，盐胁迫条件下组合的 PA 抑制了 ROS 的积累。这些结果表明，Put + Spd 显着改善了内源性 PA，通过解毒 ROS 增强了高盐胁迫耐受性。首次研究了Put与Spd协同清除ROS对C4模型谷子作物耐盐性的影响。总体而言，我们的研究结果说明了提高农艺重要作物品种耐盐性的意义。

图形摘要