Cisplatin, a platinum-based drug, is widely used in cancer treatment but is associated with significant side effects; new treatment delivery methods must be developed which enhance treatment efficiency while decreasing the sequelae of chemotherapy. The aim of this study was to develop novel liposomal nano-particles (NPs) loaded with two drugs including cisplatin and glycyrrhizic acid. This study hypothesized that introducing glycyrrhizic acid, as an adjuvant treatment, and cisplatin, as a chemotherapeutic agent, into a co-delivery system can enhance the therapeutic effect. In this study, the synthetic method and specifications of NPs were evaluated. After surface modification by polyethylene glycol (PEG), the size and the zeta potential of NPs were reported as 81.6 nm and − 30.7 mV, respectively. DLD-1 and LIM-2405 human colon cancer cell lines were then applied to evaluate the cytotoxicity of NPs using an MTT assay. The results revealed that IC₅₀ for DLD-1, as a resistant cell line to cisplatin, decreased by 48% on co-delivery system treatment. Furthermore, to determine the proliferative potential of cell lines and DNA damage on cancerous cells after treatment by NPs and cisplatin in free form, the cell proliferation assay and immunocytochemistry method were performed. The results showed that a co-delivery system caused more than 43% DNA damage to cancerous cells compared with the free form of cisplatin. The finding of this study demonstrates that the co-delivery system has a significant anti-cancer efficiency compared with the free form of cisplatin. To conclude, the results of this study demonstrate a possible application of glycyrrhizic acid as an adjuvant treatment for cisplatin on co-delivery systems.

铂基药物顺铂广泛用于癌症治疗，但具有明显的副作用。必须开发新的治疗方法，以提高治疗效率，同时减少化学疗法的后遗症。这项研究的目的是开发载有两种药物的新型脂质体纳米颗粒（NPs），其中包括顺铂和甘草酸。这项研究假设将甘草酸作为辅助治疗剂和顺铂作为化学治疗剂引入共同给药系统可以增强治疗效果。在这项研究中，对NP的合成方法和规格进行了评估。经聚乙二醇（PEG）表面修饰后，NP的大小和Zeta电位分别报告为81.6 nm和-30.7 mV。然后使用MTT分析法将DLD-1和LIM-2405人结肠癌细胞系应用于评估NP的细胞毒性。结果显示IC作为对顺铂耐药的细胞系，DLD-1的₅₀降低了共递送系统治疗的48％。此外，为了确定NPs和顺铂以游离形式处理后癌细胞系的增殖潜力和DNA对癌细胞的损伤，进行了细胞增殖测定和免疫细胞化学方法。结果显示，与游离形式的顺铂相比，共同递送系统对癌细胞造成的DNA损伤超过43％。这项研究的发现表明，与顺铂的游离形式相比，共递送系统具有显着的抗癌效率。总而言之，这项研究的结果表明甘草酸有可能作为顺铂在辅助给药系统上的辅助治疗药物。