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Comparative osteoconductivity of bone void fillers with antibiotics in a critical size bone defect model.
Journal of Materials Science: Materials in Medicine ( IF 3.7 ) Pub Date : 2020-08-25 , DOI: 10.1007/s10856-020-06418-1
Rema A Oliver 1 , Vedran Lovric 1 , Chris Christou 1 , William R Walsh 1
Affiliation  

The study aimed to evaluate the comparative osteoconductivity of three commercially available bone void fillers containing gentamicin with respect to new bone, growth, host tissue response and resorption of the implant material. Defects were created in the cancellous bone of the distal femur and proximal tibia of 12-skeletally mature sheep and filled with three commercially available bone void fillers containing gentamicin (Stimulan-G, Cerament-G, Herafill-G). Peripheral blood was taken pre-operatively and at the time of implantation, as well as at intermittent timepoints following surgery to determine systemic gentamicin levels (5-,15- and 30- minutes, 1, 2, 3, 6, 12, 24, 48- and 72-hours, 3-, 6- and 12-weeks). Decalcified, embedded samples were stained with haematoxylin and eosin (H&E) and used to assess the host tissue response and the formation of new bone in the presence of test implant materials. No adverse reactions were noted at harvest at any time points for any cancellous implantation sites with the various implant materials. Comparative microCT analysis of the Stimulan-G, Cerament-G and Herafill-G test materials revealed a similar increase in bone surface area and volume between animals implanted with Stimulan-G or Cerament-G test materials. Animals implanted with Herafill-G test materials demonstrated the lowest increases in bone volume and surface area of the test materials tested, at levels similar to the negative control sites. By 12-weeks, Stimulan-G defects were completely closed with mature bone and bone marrow whilst the Cerament-G material was still present after 12 weeks by histological examination. In conclusion, this study demonstrated differences in the bone regenerative capacity of a range of bone void fillers in an in vivo setting.



中文翻译:

在临界尺寸骨缺损模型中比较骨空隙填充物与抗生素的骨传导性。

该研究旨在评估三种市售含庆大霉素的骨空隙填充剂在新骨、生长、宿主组织反应和植入材料吸收方面的比较骨传导性。在 12 只骨骼成熟绵羊的股骨远端和胫骨近端的松质骨中产生缺陷,并用三种市售的含有庆大霉素的骨空隙填充剂(Stimulan-G、Cerament-G、Herafill-G)填充。在术前和植入时以及在手术后的间歇时间点采集外周血以确定全身庆大霉素水平(5 分钟、15 分钟和 30 分钟、1、2、3、6、12、24、 48 小时和 72 小时,3 周、6 周和 12 周)。脱钙、包埋的样品用苏木精和伊红染色(H& E) 并用于评估宿主组织的反应和在测试植入材料存在的情况下新骨的形成。对于具有各种植入材料的任何松质植入位点,在任何时间点收获时均未发现不良反应。对 Stimulan-G、Cerament-G 和 Herafill-G 测试材料的比较 microCT 分析显示,植入 Stimulan-G 或 Cerament-G 测试材料的动物之间的骨表面积和体积有类似的增加。植入 Herafill-G 测试材料的动物在与阴性对照部位相似的水平上,显示出测试材料的骨体积和表面积的最低增加。到 12 周时,通过组织学检查,Stimulan-G 缺陷完全被成熟的骨和骨髓封闭,而 Cerament-G 材料在 12 周后仍然存在。

更新日期:2020-08-25
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