To reduce excessive scarring in wound healing, electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), are fabricated for use as a wound dressing. Three PHBV polymers with different 3HV content are used to prepare either solution-cast films or electrospun nanofibrous meshes. As 3HV content increases, the crystallinity decreases and the scaffolds become more elastic. The nanofibrous meshes exhibit greater elasticity and elongation at break than films. When used to culture human dermal fibroblasts in vitro, PHBV meshes give better cell attachment and proliferation, less differentiation to myofibroblasts, and less substrate contraction. In a full-thickness mouse wound model, treatment with films or meshes enables regeneration of pale thin tissues without scabs, dehydration, or tubercular scar formation. The epidermis of wounds treated with meshes develop small invaginations in the dermis within 2 weeks, indicating hair follicle and sweat gland regeneration. Consistent with the in vitro results, meshes reduce myofibroblast differentiation in vivo through downregulation of α-SMA and TGF-β1, and upregulation of TGF-β3. The regenerated wounds treated with meshes are softer and more elastic than those treated with films. These results demonstrate that electrospun nanofibrous PHBV meshes mitigate excessive scar formation by regulating myofibroblast formation, showing their promise for use as wound dressings.

为了减少在伤口愈合过度瘢痕形成，电纺纳米纤维网，的haloarchaea生产的可生物降解的弹性体的聚（3-羟基丁酸酯组成的共（-3-羟基戊酸酯）（PHBV）被制成用作伤口敷料。使用三种具有不同3HV含量的PHBV聚合物来制备溶液流延薄膜或电纺纳米纤维网。随着3HV含量的增加，结晶度降低，支架变得更有弹性。纳米纤维网比薄膜具有更大的弹性和断裂伸长率。当用于体外培养人真皮成纤维细胞时，PHBV网片可提供更好的细胞附着和增殖，对成肌纤维细胞的分化更少，底物收缩也更少。在全厚度小鼠伤口模型中，用薄膜或网眼处理可再生苍白的薄组织，而无结ab，脱水或结节性瘢痕形成。用网眼处理的伤口的表皮在2周内在真皮中出现小块内陷，表明毛囊和汗腺再生。与体外结果一致，网孔通过下调α-SMA和TGF-β1和上调TGF-β3来减少体内成纤维细胞的分化。用网眼处理的再生伤口比用膜处理的伤口更柔软，更有弹性。这些结果表明，电纺纳米纤维PHBV网格通过调节成肌纤维细胞的形成减轻了过多的疤痕形成，表明它们有望用作伤口敷料。