Abstract

Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.

中文翻译：

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水科学中脂肪酸分析的最新技术和最佳实践

摘要

在过去的半个多世纪中，确定水生生物的脂质含量和脂肪酸（FA）组成一直是营养生态学，水产养殖和营养领域的主要工作。尽管对脂质分析的方案进行了很好的描述，但它们在水生科学中的应用通常需要进行修改以适应田间条件和样品类型。在这里，我们介绍从采样到数据处理的海洋和淡水脂质分析专用方法的当前知识水平。我们审查：（i）样品的保存，储存和运输方案及其对脂质的影响，（ii）脂质提取，极性和中性脂质的分离，衍生化和检测方法，以及（iii）可用的统计分析工具FA数据。