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Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities
Green Processing and Synthesis ( IF 4.3 ) Pub Date : 2020-08-20 , DOI: 10.1515/gps-2020-0047
Abid Hussain 1 , Ansar Mehmood 1 , Ghulam Murtaza 2 , Khawaja Shafique Ahmad 1 , Aneela Ulfat 1 , Muhammad Faraz Khan 1 , Tariq Saif Ullah 3
Affiliation  

Abstract In this study, we reported an easy, rapid, cost-effective and environmentally benign method for the fabrication of silver nanoparticles (Ag-NPs) using Olea ferruginea as reducing, capping and stabilizing agent. For this, an aqueous extract of leaf and bark of O. ferruginea was treated with 1 mM AgNO3, which reduces Ag ions to Ag-NPs by establishing reddish brown color. The synthesized Ag-NPs were spherical crystals, with a mean size of 23 and 17 nm for leaf- and bark-mediated Ag-NPs, respectively. Fourier transform infrared spectroscopy affirmed the role of leaf and bark extracts of O. ferruginea as reducing, capping and stabilizing agent. These biosynthesized Ag-NPs showed profound antibacterial activity against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Streptococcus pneumonia and Staphylococcus aureus) bacteria. The highest antibacterial activity was shown by bark Ag-NPs against S. aureus (14.00 mm), while leaf Ag-NPs showed higher activity against S. pneumonia (13.00 mm). Additionally, they produced effective antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) as compared to plant extracts and positive control. It was observed that the bark-mediated Ag-NPs had higher percentage (90%) of scavenging potential than the leaf-mediated Ag-NPs (78%). The significance of the current study is the synthesis of eco-friendly, easy and cost-effective Ag-NPs as biomedical products.

中文翻译:

使用 Olea ferruginea Royle 进行抗菌和抗氧化活性的银纳米粒子的环境友好合成和表征

摘要 在这项研究中,我们报道了一种使用铁橄榄作为还原剂、封端剂和稳定剂制备银纳米粒子 (Ag-NPs) 的简单、快速、经济高效且环境友好的方法。为此,用 1 mM AgNO3 处理了 O. ferruginea 叶和树皮的水提取物,通过建立红棕色将 Ag 离子还原为 Ag-NPs。合成的 Ag-NPs 是球形晶体,叶和树皮介导的 Ag-NPs 的平均尺寸分​​别为 23 和 17 nm。傅里叶变换红外光谱证实了 O. ferruginea 的叶和树皮提取物作为还原剂、加盖剂和稳定剂的作用。这些生物合成的 Ag-NPs 对革兰氏阴性菌(铜绿假单胞菌和大肠杆菌)和革兰氏阳性菌(肺炎链球菌和金黄色葡萄球菌)显示出强大的抗菌活性。树皮 Ag-NPs 对金黄色葡萄球菌 (14.00 mm) 的抗菌活性最高,而叶 Ag-NPs 对肺炎链球菌 (13.00 mm) 表现出更高的活性。此外,与植物提取物和阳性对照相比,它们对 2,2-二苯基-1-苦基肼 (DPPH) 产生了有效的抗氧化活性。据观察,树皮介导的 Ag-NPs 具有比叶介导的 Ag-NPs (78%) 更高的清除潜力百分比 (90%)。目前研究的意义在于合成环保、简单且具有成本效益的 Ag-NPs 作为生物医学产品。树皮 Ag-NPs 对金黄色葡萄球菌 (14.00 mm) 的抗菌活性最高,而叶 Ag-NPs 对肺炎链球菌 (13.00 mm) 表现出更高的活性。此外,与植物提取物和阳性对照相比,它们对 2,2-二苯基-1-苦基肼 (DPPH) 产生了有效的抗氧化活性。据观察,树皮介导的 Ag-NPs 具有比叶介导的 Ag-NPs (78%) 更高的清除潜力百分比 (90%)。目前研究的意义在于合成环保、简单且具有成本效益的 Ag-NPs 作为生物医学产品。树皮 Ag-NPs 对金黄色葡萄球菌 (14.00 mm) 的抗菌活性最高,而叶 Ag-NPs 对肺炎链球菌 (13.00 mm) 表现出更高的活性。此外,与植物提取物和阳性对照相比,它们对 2,2-二苯基-1-苦基肼 (DPPH) 产生了有效的抗氧化活性。据观察,树皮介导的 Ag-NPs 具有比叶介导的 Ag-NPs (78%) 更高的清除潜力百分比 (90%)。当前研究的意义在于合成环保、简单且具有成本效益的 Ag-NPs 作为生物医学产品。据观察,树皮介导的 Ag-NPs 具有比叶介导的 Ag-NPs (78%) 更高的清除潜力百分比 (90%)。当前研究的意义在于合成环保、简单且具有成本效益的 Ag-NPs 作为生物医学产品。据观察,树皮介导的 Ag-NPs 具有比叶介导的 Ag-NPs (78%) 更高的清除潜力百分比 (90%)。目前研究的意义在于合成环保、简单且具有成本效益的 Ag-NPs 作为生物医学产品。
更新日期:2020-08-20
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