ABSTRACT Calcium-dependent protein kinases-related kinases (CDPK-related kinases; CRKs) are Ser/Thr kinases that bind with Ca2+/Calmodulin and play crucial roles in signal transduction pathways during plant growth, development, and responses to multiple stresses. In this study, we have studied detailed organ and tissue-specific expression patterns of rice CRK genes. Our organ-specific RT-PCR analyzes show the differential expression pattern of these genes in various organs of rice. Moreover, our RNA-RNA in situ hybridization study in rice stem base containing developing crown root primordia demonstrates that the expression of CRK genes is spatially restricted to the developing crown root primordia, suggesting their putative role in protein phosphorylation-dependent cellular signaling during rice crown root development. Furthermore, organ-specific differentially expression pattern of CRK genes during floral organogenesis further support for the organ-specific cell signaling during organogenesis. Thus, our study provides a developmentally regulated expression pattern of rice CRK genes, though they are broadly expressed and a basic foundation for functional characterizations of CRK gene members to unravel their specific functions during plant growth and development.

中文翻译：

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水稻中钙依赖性蛋白激酶相关激酶（CRKs）的组织特异性表达模式

摘要 钙依赖性蛋白激酶相关激酶（CDPK 相关激酶；CRK）是 Ser/Thr 激酶，可与 Ca2+/钙调蛋白结合，在植物生长、发育和对多种胁迫的反应过程中的信号转导途径中起关键作用。在这项研究中，我们研究了水稻 CRK 基因的详细器官和组织特异性表达模式。我们的器官特异性 RT-PCR 分析显示了这些基因在水稻不同器官中的差异表达模式。此外，我们在含有发育中冠根原基的水稻茎基中进行的 RNA-RNA 原位杂交研究表明，CRK 基因的表达在空间上仅限于发育中的冠根原基，表明它们在水稻冠过程中蛋白质磷酸化依赖性细胞信号传导中的推定作用。根发育。此外，花器官发生过程中 CRK 基因的器官特异性差异表达模式进一步支持器官发生过程中器官特异性细胞信号传导。因此，我们的研究提供了水稻 CRK 基因的发育调控表达模式，尽管它们广泛表达，并且为 CRK 基因成员的功能表征以揭示其在植物生长和发育过程中的特定功能奠定了基础。