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Embryo growth and seed germination of four taxa of Narcissus (section Pseudonarcissi) conserved under non‐recalcitrant seed conditions in germplasm banks
Plant Species Biology ( IF 1.4 ) Pub Date : 2020-08-23 , DOI: 10.1111/1442-1984.12288
José M. Herranz Sanz 1, 2 , Miguel A. Copete Carreño 1, 2 , Raquel Herranz Ferrer 1 , Alejandro Santiago González 2, 3 , Elena Copete Carreño 1, 2 , Pablo Ferrandis Gotor 1, 2, 3
Affiliation  

The aim of this study was to determine the germinative ability of the seeds of four Narcissus taxa belonging to Section Pseudonarcissi after they had been conserved under the conditions of non‐recalcitrant seed storage protocols. For each taxon (N. alcaracensis, N. longispathus, N. radinganorum and N. pseudonarcissus subsp. munozii‐garmendiae), one seed lot was desiccated to 4% moisture content (MC) and stored under laboratory conditions (22°C, 40–50% relative humidity (RH), whereas another was dehydrated to 3% MC and stored at −10°C. The latter treatment simulated standard conservation conditions for non‐recalcitrant seeds. After 26 months, embryo growth and germination were evaluated. Seed responses were correlated with their MC upon dispersal. Seeds of N. alcaracensis, N. longispathus and N. radinganorum left to dry on the mother plant during maturation had 8–10% MC when dispersed, tolerated non‐recalcitrant seed conservation and germinated to >90% under the most favorable incubation conditions. Narcissus pseudonarcissus subsp. munozii‐garmendiae seeds did not undergo maturation drying and had 46.7% MC upon dispersal. They reached 100% germination after being desiccated to 4% and stored at 22°C, were not recalcitrant, but failed to germinate when stored at −10°C under non‐recalcitrant seed conservation conditions. Therefore, N. alcaracensis, N. longispathus and N. radinganorum seeds can be conserved under non‐recalcitrant seed conditions in germplasm banks, whereas those of N. pseudonarcissus subsp. munozii‐garmendiae are moderately recalcitrant. Seed storage behavior is influenced primarily by the extent of maturation drying of the seeds on the mother plant.

中文翻译:

在非顽固性种子条件下在种质库中保存的四个水仙(Pseudonarcissi节)的类群的胚胎生长和种子萌发

这项研究的目的是确定在非顽固性种子储存方案条件下保存后,属于水仙科的四个水仙类群的种子的萌发能力。对于每一个分类单元(N. alcaracensisN. longispathusN. radinganorumN. pseudonarcissus亚种munozii-garmendiae),将一粒种子干燥至4%的水分含量(MC)并在实验室条件下(22°C,40–50%相对湿度(RH))存储,而另一种子脱水至3%MC并存储在-10°C后一种处理模拟了非顽it型种子的标准保存条件,经过26个月后,评估了胚的生长和萌发,种子的反应与其分散后的MC相关,N。alcaracensisN。longispathusN. radinganorum的种子留给了种子成熟时在母本植物上干燥时,分散时的MC为8-10%,可以忍受不顽固的种子,并且在最有利的孵化条件下发芽到> 90%的水仙拟水仙亚种munozii-garmendiae种子没有进行成熟干燥,分散时的MC为46.7%。将其干燥至4%并在22°C储存后达到100%的发芽率,不顽固,但在非顽固的种子保存条件下在-10°C储存时无法发芽。因此,N. alcaracensisN. longispathusN. radinganorum种子可以在种质库非顽拗种子的条件下是保守的,而那些的N. pseudonarcissus亚种。munozii-garmendiae是中度顽固的。种子贮藏行为主要受母植物上种子成熟干燥程度的影响。
更新日期:2020-10-07
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