NMR spectroscopy is integral to metabolic studies, yet it can suffer from the long acquisition times required to collect data of sufficient signal strength and resolution. The use of non-uniform sampling (NUS) allows faster collection of NMR spectra without loss of spectral integrity. When planning experimental methodologies to perform metabolic flux analysis (MFA) of cell metabolism, a variety of options are available for the acquisition of NUS NMR data. Before beginning data collection, decisions have to be made regarding selection of pulse sequence, number of transients and NUS specific parameters such as the sampling level and sampling schedule. Poor choices will impact data quality, which may have a negative effect on the subsequent analysis and biological interpretation. Herein, we describe factors that should be considered when setting up non-uniformly sampled 2D-1 H,13 C HSQC NMR experiments for MFA and provide a standard protocol for users to follow.

中文翻译：

---

用于代谢通量分析的非均匀采样 2D-HSQC NMR 谱的优化收集

核磁共振光谱是代谢研究不可或缺的一部分，但它可能会受到收集足够信号强度和分辨率数据所需的较长采集时间的影响。使用非均匀采样 (NUS) 可以更快地收集 NMR 光谱，而不会损失光谱完整性。在规划对细胞代谢进行代谢通量分析 (MFA) 的实验方法时，有多种选项可用于采集 NUS NMR 数据。在开始数据收集之前，必须就脉冲序列的选择、瞬变次数和 NUS 特定参数（例如采样水平和采样计划）做出决定。糟糕的选择会影响数据质量，这可能会对后续分析和生物学解释产生负面影响。在此处，