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Pinpointing oxidative stress behind the White Striping myopathy: Depletion of antioxidant defenses, accretion of oxidized proteins and impaired proteostasis
Journal of the Science of Food and Agriculture ( IF 4.1 ) Pub Date : 2020-08-24 , DOI: 10.1002/jsfa.10747
Leila M Carvalho 1 , Josué Delgado 2 , Marta S Madruga 1 , Mario Estévez 3
Affiliation  

BACKGROUND This study aimed to investigate the molecular mechanisms involved in the onset of the White Striping (WS) myopathy with particular attention to the role of oxidative stress and protein oxidation in the loss of meat quality. RESULTS WS-M (moderate degree; white stripes <1 mm thickness) and WS-S (severe degree; white stripes >1 mm thickness) breasts presented higher pH, hardness, redness, lipid, and collagen content, and lower lightness compared to the Normal breast. Compared to the latter, WS-S had a more severe loss of protein thiols (70.7% less thiols than in N), reduced activity of antioxidant enzymes such as catalase (23 vs 40 U/g), glutathione peroxidase (0.21 vs 0.54 U/g) and superoxide dismutase (56 vs 73 U/g), and consequently, had greater accretion of thiobarbituric acidreactive substances (0.64 vs 0.22 mg MDA/kg muscle), allysine (3.1 vs 1.9 nmol/mg protein) and Schiff base structures (645 vs 258 fluorescent units). The analysis of sarcoplasmic proteins revealed that muscles severely affected by the myopathy suffered a chronic impairment of physiological (upregulation of sarcoplasmic reticulum Ca2+ ATPase, sarcalumenin and calsequestrin-2) and metabolic processes (downregulation of pyruvate kinase, creatine kinase and L-lactate dehydrogenase). CONCLUSION The overexpression of ribonuclease/angiogenin inhibitor 1 and Kelch-like proteins in WS chicken breasts indicates altered protein turnover plausibly mediated by oxidative stress and accumulation of oxidized proteins. This article is protected by copyright. All rights reserved.

中文翻译:

查明白色条纹肌病背后的氧化应激:抗氧化防御的消耗、氧化蛋白的增加和蛋白质稳态受损

背景本研究旨在研究白条纹(WS)肌病发病的分子机制,特别关注氧化应激和蛋白质氧化在肉质损失中的作用。结果 WS-M(中等程度;白色条纹 <1 mm 厚)和 WS-S(严重程度;白色条纹 >1 mm 厚)乳房呈现出更高的 pH、硬度、发红、脂质和胶原蛋白含量,与正常的乳房。与后者相比,WS-S 的蛋白质硫醇损失更严重(硫醇比 N 少 70.7%),抗氧化酶的活性降低,如过氧化氢酶(23 对 40 U/g)、谷胱甘肽过氧化物酶(0.21 对 0.54 U/g) /g) 和超氧化物歧化酶(56 对 73 U/g),因此,硫代巴比妥酸反应性物质的增加更多(0.64 对 0.22 毫克 MDA/kg 肌肉),赖氨酸(3.1 对 1.9 nmol/mg 蛋白质)和希夫碱结构(645 对 258 荧光单位)。肌浆蛋白分析显示,受肌病严重影响的肌肉遭受生理(肌质网 Ca2+ ATPase、sarcalumenin 和 calsequestrin-2 上调)和代谢过程(丙酮酸激酶、肌酸激酶和 L-乳酸脱氢酶下调)的慢性损伤. 结论 WS 鸡胸肉中核糖核酸酶/血管生成素抑制剂 1 和 Kelch 样蛋白的过度表达表明蛋白质周转的改变可能由氧化应激和氧化蛋白的积累介导。本文受版权保护。版权所有。肌浆蛋白分析显示,受肌病严重影响的肌肉遭受生理(肌质网 Ca2+ ATPase、sarcalumenin 和 calsequestrin-2 上调)和代谢过程(丙酮酸激酶、肌酸激酶和 L-乳酸脱氢酶下调)的慢性损伤. 结论 WS 鸡胸肉中核糖核酸酶/血管生成素抑制剂 1 和 Kelch 样蛋白的过度表达表明蛋白质周转的改变可能由氧化应激和氧化蛋白的积累介导。本文受版权保护。版权所有。肌浆蛋白分析显示,受肌病严重影响的肌肉遭受生理(肌质网 Ca2+ ATPase、sarcalumenin 和 calsequestrin-2 上调)和代谢过程(丙酮酸激酶、肌酸激酶和 L-乳酸脱氢酶下调)的慢性损伤. 结论 WS 鸡胸肉中核糖核酸酶/血管生成素抑制剂 1 和 Kelch 样蛋白的过度表达表明蛋白质周转的改变可能由氧化应激和氧化蛋白的积累介导。本文受版权保护。版权所有。sarcalumenin 和 calsequestrin-2)和代谢过程(丙酮酸激酶、肌酸激酶和 L-乳酸脱氢酶的下调)。结论 WS 鸡胸肉中核糖核酸酶/血管生成素抑制剂 1 和 Kelch 样蛋白的过度表达表明蛋白质周转的改变可能由氧化应激和氧化蛋白的积累介导。本文受版权保护。版权所有。sarcalumenin 和 calsequestrin-2)和代谢过程(丙酮酸激酶、肌酸激酶和 L-乳酸脱氢酶的下调)。结论 WS 鸡胸肉中核糖核酸酶/血管生成素抑制剂 1 和 Kelch 样蛋白的过度表达表明蛋白质周转的改变可能由氧化应激和氧化蛋白的积累介导。本文受版权保护。版权所有。
更新日期:2020-08-24
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