To assess the effect of biodentine (BD) and MTA‐angelus (MTA) on biocompatibility, BMP2, BMP4, and osteocalcin (OC) expression. Subcutaneously implanted tubes of four groups (MTA, BD, Control, and Sham) were kept over 15, 30, and 60 days; histological analyses were performed using H&E and Von Kossa; ELISA quantified IL‐1β and IL‐8 expression; and qRT‐PCR verified gene expression of BMPs and OC. Sham showed slight changes in profile/intensity of inflammatory infiltrate in all periods. Control had an inflammatory score significantly higher than Sham at 15 days (p < .05). BD revealed a similar inflammatory response to Sham, without significant changes over periods. MTA group exhibited an increase in chronic inflammatory profile at 30 days, with significant reduction at 60 days, when compared to Sham (p < .05). At 30/60 days, experimental groups presented birefringent areas. At 30/60 days, BD and MTA significantly increase IL‐1β compared to Control, whereas an increase in IL‐8 was observed only in BD. At 30/60 days, BD produces an expression of BMP2 whereas MTA influenced BMP4 and OC. Materials tested are biocompatible and they have osteoinductive activity; the materials influenced the expression of the tested mediators differently, suggesting different affinities with the substrate and the dental substrates.

中文翻译：

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两种牙髓修复水泥的生物矿化中的骨形态发生蛋白。

评估生物牙本质 (BD) 和 MTA-当归 (MTA) 对生物相容性、BMP2、BMP4 和骨钙素 (OC) 表达的影响。四组（MTA、BD、Control 和 Sham）皮下植入管分别保存 15、30 和 60 天；使用 H&E 和 Von Kossa 进行组织学分析；ELISA 定量 IL-1β 和 IL-8 表达；和 qRT-PCR 验证了 BMP 和 OC 的基因表达。假手术在所有时期显示炎症浸润的分布/强度略有变化。对照组在 15 天时的炎症评分显着高于 Sham ( p  < .05)。BD 显示出与 Sham 相似的炎症反应，但在一段时间内没有显着变化。与 Sham 相比，MTA 组在 30 天时慢性炎症特征增加，在 60 天时显着降低。p  < .05)。在 30/60 天，实验组呈现双折射区域。在 30/60 天，与对照相比，BD 和 MTA 显着增加 IL-1β，而仅在 BD 中观察到 IL-8 增加。在 30/60 天，BD 产生 BMP2 的表达，而 MTA 影响 BMP4 和 OC。测试的材料具有生物相容性，并且具有骨诱导活性；材料对测试介质的表达产生不同的影响，表明与基材和牙科基材的亲和力不同。